Detailed information of TA system TA023084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 41379..42053
• Replicon: plasmid pKP91
• Accession: NC_011281
• Organism: Klebsiella pneumoniae 342

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KPK_RS27620
• Protein ID: WP_012539985.1
• Coordinates: 41730..42053 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPK_RS27615
• Protein ID: WP_012540018.1
• Coordinates: 41379..41681 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPK_RS27590	37085..37606	+	522	WP_041165526.1	hypothetical protein	-
KPK_RS27595	37708..38130	-	423	Protein_35	SOS response-associated peptidase family protein	-
KPK_RS27600	38527..38946	+	420	WP_012540023.1	ASCH domain-containing protein	-
KPK_RS29855	39475..39636	+	162	Protein_37	transposase	-
KPK_RS29860	39862..40197	+	336	WP_032700858.1	TonB family protein	-
KPK_RS27605	40222..40764	+	543	WP_012539969.1	GNAT family N-acetyltransferase	-
KPK_RS27610	41053..41343	+	291	WP_012540036.1	hypothetical protein	-
KPK_RS27615	41379..41681	-	303	WP_012540018.1	helix-turn-helix domain-containing protein	Antitoxin
KPK_RS27620	41730..42053	-	324	WP_012539985.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPK_RS27625	42152..42829	+	678	Protein_43	IS110 family transposase	-
KPK_RS27630	42966..43592	-	627	WP_170873325.1	peptidase	-
KPK_RS27635	44095..45090	+	996	WP_012539965.1	IS110 family transposase	-
KPK_RS27640	45211..46005	-	795	WP_012540031.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91096	91096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (45-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-83)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12358.27 Da
• Isoelectric Point: 9.6240

>T23084 WP_012539985.1 NC_011281:c42053-41730 [Klebsiella variicola]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDDLSEKEKAVMKALTQQLK

Nucleotide

• Length: 324 bp

>T23084 NC_011281:c42053-41730 [Klebsiella variicola]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAGCTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACAATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GGCGCCCGGGGATGGGTAAGCGAGGTGGGGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGATCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11226.13 Da
• Isoelectric Point: 6.8718

>AT23084 WP_012540018.1 NC_011281:c41681-41379 [Klebsiella variicola]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRIPSGIALK
MLCLIEREPALIDTLRGIHV

Nucleotide

• Length: 303 bp

>AT23084 NC_011281:c41681-41379 [Klebsiella variicola]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGTGCCAGCGTTGACCTTGTCCGCAGCTGGGAACAGCAACGGCGTATTCCGTCGGGTATAGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTGATACGTTGCGTGGTATTCACGTCTGA