Detailed information of TA system TA230826

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 102968..103237
• Replicon: plasmid pB379_3
• Accession: NZ_CP090458
• Organism: Escherichia coli strain B379

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LZ684_RS24435
• Protein ID: WP_001372321.1
• Coordinates: 103112..103237 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	102968..103033 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ684_RS24400	98678..99205	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LZ684_RS24405	99263..99496	+	234	WP_000006003.1	DUF905 family protein	-
LZ684_RS24410	99557..101580	+	2024	Protein_114	ParB/RepB/Spo0J family partition protein	-
LZ684_RS24415	101649..102083	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LZ684_RS24420	102080..102842	+	763	Protein_116	plasmid SOS inhibition protein A	-
-	102811..103035	+	225	NuclAT_0	-	-
-	102811..103035	+	225	NuclAT_0	-	-
-	102811..103035	+	225	NuclAT_0	-	-
-	102811..103035	+	225	NuclAT_0	-	-
LZ684_RS24425	102820..102999	-	180	WP_001309233.1	hypothetical protein	-
-	102968..103033	-	66	-	-	Antitoxin
LZ684_RS24430	103021..103170	+	150	Protein_118	plasmid maintenance protein Mok	-
LZ684_RS24435	103112..103237	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LZ684_RS24440	103719..105290	-	1572	WP_000381395.1	IS66-like element ISCro1 family transposase	-
LZ684_RS24445	105310..105657	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
LZ684_RS24450	105657..106334	-	678	WP_001682408.1	IS66-like element accessory protein TnpA	-
LZ684_RS24455	106393..106559	-	167	Protein_123	hypothetical protein	-
LZ684_RS24460	106859..107155	+	297	WP_001272251.1	hypothetical protein	-
LZ684_RS24465	107266..108087	+	822	WP_001234445.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / blaCTX-M-14 / qacE / cmlA1 / sul2 / floR / erm(42) / ant(3'')-Ia / sitABCD	iucA / iucB / iucC / iucD / iutA	1..147204	147204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T230826 WP_001372321.1 NZ_CP090458:103112-103237 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T230826 NZ_CP125620:366004-366306 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT230826 NZ_CP090458:c103033-102968 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin