Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 3749613..3750307 | Replicon | chromosome |
| Accession | NZ_CP090456 | ||
| Organism | Escherichia coli strain B379 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | S1EXB8 |
| Locus tag | LZ684_RS18085 | Protein ID | WP_001263493.1 |
| Coordinates | 3749613..3750011 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1FJN6 |
| Locus tag | LZ684_RS18090 | Protein ID | WP_000554757.1 |
| Coordinates | 3750014..3750307 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (3745273) | 3745273..3745353 | - | 81 | NuclAT_8 | - | - |
| - (3745273) | 3745273..3745353 | - | 81 | NuclAT_8 | - | - |
| - (3745273) | 3745273..3745353 | - | 81 | NuclAT_8 | - | - |
| - (3745273) | 3745273..3745353 | - | 81 | NuclAT_8 | - | - |
| LZ684_RS18055 (3744613) | 3744613..3745857 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| LZ684_RS18060 (3745949) | 3745949..3746407 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| LZ684_RS18065 (3746668) | 3746668..3748125 | + | 1458 | WP_001293003.1 | cytosol nonspecific dipeptidase | - |
| LZ684_RS18070 (3748182) | 3748182..3748703 | - | 522 | Protein_3539 | peptide chain release factor H | - |
| LZ684_RS18075 (3748702) | 3748702..3748905 | - | 204 | Protein_3540 | RtcB family protein | - |
| LZ684_RS18080 (3749151) | 3749151..3749603 | - | 453 | WP_001059847.1 | GNAT family N-acetyltransferase | - |
| LZ684_RS18085 (3749613) | 3749613..3750011 | - | 399 | WP_001263493.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| LZ684_RS18090 (3750014) | 3750014..3750307 | - | 294 | WP_000554757.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| LZ684_RS18095 (3750359) | 3750359..3751414 | - | 1056 | WP_001226164.1 | DNA polymerase IV | - |
| LZ684_RS18100 (3751485) | 3751485..3752270 | - | 786 | WP_000207587.1 | putative lateral flagellar export/assembly protein LafU | - |
| LZ684_RS18105 (3752242) | 3752242..3753954 | + | 1713 | Protein_3546 | flagellar biosynthesis protein FlhA | - |
| LZ684_RS18110 (3754170) | 3754170..3754667 | - | 498 | WP_000006256.1 | REP-associated tyrosine transposase RayT | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | dfrA1 / ant(3'')-Ia / qacE / sul1 / tet(B) / cmlA1 / sul3 / blaTEM-1B | fdeC / fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / gmhA/lpcA | 3677614..3764877 | 87263 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15472.83 Da Isoelectric Point: 8.0949
>T230817 WP_001263493.1 NZ_CP090456:c3750011-3749613 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T230817 NZ_CP125619:c325916-325509 [Pseudomonas sp. PMCC200367]
ATGATCAAATACATGCTCGATACCAACATCTGCATCTTCACCATCAAGAACAAACCGCAAATCGTACGAGAAGCTTTCAA
TCGTCATAACGGGCAGCTATGCATCAGTGCCGTCACGCTGATGGAGTTGTTCTACGGCGCCGAAAAATCGGCCGCACCGG
AAAAGAACCTCGCGGTGATCGAGGGTTTTGTGGCACGCCTTGAGGTCTTGCCTTTCGACAATGAAGCGGCTGCGCACACC
GGTATGATTCGATCCGAGTTGGCCAAGGACGGAACGCCTATCGGGCCCTACGACCACATGATTGCCGGCCACGCACGTTC
ACGCGGATTCATAGTGGTCACCAACAACCTACGCGAATTTGATCGGGTTCCAGGGCTACGCGTAGAAGACTGGGTTCACC
CCGATTGA
ATGATCAAATACATGCTCGATACCAACATCTGCATCTTCACCATCAAGAACAAACCGCAAATCGTACGAGAAGCTTTCAA
TCGTCATAACGGGCAGCTATGCATCAGTGCCGTCACGCTGATGGAGTTGTTCTACGGCGCCGAAAAATCGGCCGCACCGG
AAAAGAACCTCGCGGTGATCGAGGGTTTTGTGGCACGCCTTGAGGTCTTGCCTTTCGACAATGAAGCGGCTGCGCACACC
GGTATGATTCGATCCGAGTTGGCCAAGGACGGAACGCCTATCGGGCCCTACGACCACATGATTGCCGGCCACGCACGTTC
ACGCGGATTCATAGTGGTCACCAACAACCTACGCGAATTTGATCGGGTTCCAGGGCTACGCGTAGAAGACTGGGTTCACC
CCGATTGA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.81 Da Isoelectric Point: 5.9050
>AT230817 WP_000554757.1 NZ_CP090456:c3750307-3750014 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT230817 NZ_CP125619:c326146-325916 [Pseudomonas sp. PMCC200367]
ATGGAACAGACCACCCTTTTCATGAGCAATCGAAGCCAGGCTGTCCGCCTCCCCAAAGCTGTTGCCATGCCAAGCGACGT
GAAACGAGTCAACGTAGTTGCCATCGGCAGGGCTCGCATCATCACTCCCGCAGATGAAACATGGGACAGCTGGTTTGACG
GGGATGGCGTAAGTGCAGATTTCATGACCGATCGTGAACAACCCGCCGATCAGGAGCGTGAGTCGTTCTGA
ATGGAACAGACCACCCTTTTCATGAGCAATCGAAGCCAGGCTGTCCGCCTCCCCAAAGCTGTTGCCATGCCAAGCGACGT
GAAACGAGTCAACGTAGTTGCCATCGGCAGGGCTCGCATCATCACTCCCGCAGATGAAACATGGGACAGCTGGTTTGACG
GGGATGGCGTAAGTGCAGATTTCATGACCGATCGTGAACAACCCGCCGATCAGGAGCGTGAGTCGTTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|