Detailed information of TA system TA230787

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 3799..4068
• Replicon: plasmid pPT62-tetX-108kb
• Accession: NZ_CP090449
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LZ175_RS22255
• Protein ID: WP_001372321.1
• Coordinates: 3943..4068 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	3799..3864 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS22225 (169)	169..402	+	234	WP_000005987.1	DUF905 family protein	-
LZ175_RS22230 (467)	467..2425	+	1959	WP_039000998.1	ParB/RepB/Spo0J family partition protein	-
LZ175_RS22235 (2480)	2480..2914	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LZ175_RS22240 (2911)	2911..3673	+	763	Protein_4	plasmid SOS inhibition protein A	-
LZ175_RS22245 (3642)	3642..3830	-	189	WP_001299721.1	hypothetical protein	-
- (3799)	3799..3864	+	66	NuclAT_1	-	-
- (3799)	3799..3864	-	66	NuclAT_0	-	Antitoxin
- (3799)	3799..3864	-	66	NuclAT_0	-	Antitoxin
- (3642)	3642..3866	+	225	NuclAT_0	-	-
- (3642)	3642..3866	+	225	NuclAT_0	-	-
- (3642)	3642..3866	+	225	NuclAT_0	-	-
- (3642)	3642..3866	+	225	NuclAT_0	-	-
- (3642)	3642..3866	-	225	NuclAT_0	-	-
LZ175_RS22250 (3852)	3852..4001	+	150	Protein_6	plasmid maintenance protein Mok	-
LZ175_RS22255 (3943)	3943..4068	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LZ175_RS22260 (4288)	4288..4518	+	231	WP_001426396.1	hypothetical protein	-
LZ175_RS22265 (4516)	4516..4689	-	174	Protein_9	hypothetical protein	-
LZ175_RS22270 (4759)	4759..4965	+	207	WP_000547968.1	hypothetical protein	-
LZ175_RS22275 (4990)	4990..5277	+	288	WP_000107535.1	hypothetical protein	-
LZ175_RS22280 (5399)	5399..6220	+	822	WP_001234469.1	DUF932 domain-containing protein	-
LZ175_RS22285 (6517)	6517..7119	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
LZ175_RS22290 (7440)	7440..7823	+	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LZ175_RS22295 (8010)	8010..8699	+	690	WP_039002187.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T230787 WP_001372321.1 NZ_CP090449:3943-4068 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T230787 NZ_CP125363:c5745453-5745154 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT230787 NZ_CP090449:c3864-3799 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin