Detailed information of TA system TA230786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4382679..4383281
• Replicon: chromosome
• Accession: NZ_CP090448
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LZ175_RS21300
• Protein ID: WP_000897305.1
• Coordinates: 4382970..4383281 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZ175_RS21295
• Protein ID: WP_000356397.1
• Coordinates: 4382679..4382969 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS21270 (4378607)	4378607..4379509	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LZ175_RS21275 (4379506)	4379506..4380141	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LZ175_RS21280 (4380138)	4380138..4381067	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LZ175_RS21285 (4381396)	4381396..4381638	-	243	WP_024199136.1	CopG family transcriptional regulator	-
LZ175_RS21290 (4381857)	4381857..4382075	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LZ175_RS21295 (4382679)	4382679..4382969	-	291	WP_000356397.1	NadS family protein	Antitoxin
LZ175_RS21300 (4382970)	4382970..4383281	-	312	WP_000897305.1	hypothetical protein	Toxin
LZ175_RS21305 (4383510)	4383510..4384418	+	909	WP_001491417.1	alpha/beta hydrolase	-
LZ175_RS21310 (4384587)	4384587..4385501	-	915	Protein_4162	transposase	-
LZ175_RS21315 (4385514)	4385514..4386401	-	888	Protein_4163	hypothetical protein	-
LZ175_RS21320 (4386811)	4386811..4387752	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LZ175_RS21325 (4387797)	4387797..4388234	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T230786 WP_000897305.1 NZ_CP090448:c4383281-4382970 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T230786 NZ_CP125363:c5745157-5744867 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT230786 WP_000356397.1 NZ_CP090448:c4382969-4382679 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT230786 NZ_CP125363:c5745453-5745154 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7