230780

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3621862..3622403 Replicon chromosome
Accession NZ_CP090448
Organism Escherichia coli strain PT62

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag LZ175_RS17725 Protein ID WP_000615980.1
Coordinates 3622125..3622403 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag LZ175_RS17720 Protein ID WP_000729709.1
Coordinates 3621862..3622122 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZ175_RS17700 (3617638) 3617638..3618423 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
LZ175_RS17705 (3618395) 3618395..3620107 + 1713 Protein_3468 flagellar biosynthesis protein FlhA -
LZ175_RS17710 (3620230) 3620230..3620676 - 447 WP_225520033.1 transposase -
LZ175_RS17715 (3620903) 3620903..3621652 - 750 WP_001695461.1 C40 family peptidase -
LZ175_RS17720 (3621862) 3621862..3622122 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LZ175_RS17725 (3622125) 3622125..3622403 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LZ175_RS17730 (3622559) 3622559..3623299 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LZ175_RS17735 (3623270) 3623270..3624037 - 768 WP_000333380.1 class II glutamine amidotransferase -
LZ175_RS17740 (3624243) 3624243..3624821 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T230780 WP_000615980.1 NZ_CP090448:3622125-3622403 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T230780 NZ_CP090448:3622125-3622403 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT230780 WP_000729709.1 NZ_CP090448:3621862-3622122 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT230780 NZ_CP090448:3621862-3622122 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCTTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References