Detailed information of TA system TA230766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1812068..1812570
• Replicon: chromosome
• Accession: NZ_CP090448
• Organism: Escherichia coli strain PT62

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: LZ175_RS08635
• Protein ID: WP_000767829.1
• Coordinates: 1812316..1812570 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: LZ175_RS08630
• Protein ID: WP_001259255.1
• Coordinates: 1812068..1812319 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ175_RS08605 (1807246)	1807246..1808313	-	1068	WP_000080134.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
LZ175_RS08610 (1808313)	1808313..1809383	-	1071	WP_000108952.1	histidinol-phosphate transaminase	-
LZ175_RS08615 (1809380)	1809380..1810684	-	1305	WP_001300646.1	histidinol dehydrogenase	-
LZ175_RS08620 (1810690)	1810690..1811589	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
LZ175_RS08625 (1811735)	1811735..1811785	-	51	WP_001364200.1	his operon leader peptide	-
LZ175_RS08630 (1812068)	1812068..1812319	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LZ175_RS08635 (1812316)	1812316..1812570	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
LZ175_RS08640 (1812653)	1812653..1813477	+	825	WP_000754749.1	SDR family oxidoreductase	-
LZ175_RS08645 (1813523)	1813523..1814452	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
LZ175_RS08650 (1814667)	1814667..1814729	+	63	WP_010723108.1	membrane protein YoeI	-
LZ175_RS08655 (1814719)	1814719..1816077	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
LZ175_RS08660 (1816256)	1816256..1817314	+	1059	WP_230069303.1	thiosulfate utilization transporter TsuA/YeeE	-
LZ175_RS08665 (1817328)	1817328..1817555	+	228	WP_050867712.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T230766 WP_000767829.1 NZ_CP090448:1812316-1812570 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T230766 NZ_CP125358:c29744-29496 [Escherichia fergusonii]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT230766 WP_001259255.1 NZ_CP090448:1812068-1812319 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT230766 NZ_CP125358:c29499-29152 [Escherichia fergusonii]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0