Detailed information of TA system TA230753

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 133777..134030
• Replicon: plasmid pB
• Accession: NZ_CP090434
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain KPN857

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LZ608_RS28715
• Protein ID: WP_001312851.1
• Coordinates: 133881..134030 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	133777..133836 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ608_RS28675 (129159)	129159..129503	-	345	Protein_162	IS6-like element IS26 family transposase	-
LZ608_RS28680 (129555)	129555..130259	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LZ608_RS28685 (130284)	130284..130484	+	201	WP_072354025.1	hypothetical protein	-
LZ608_RS28690 (130504)	130504..131250	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LZ608_RS28695 (131305)	131305..131865	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LZ608_RS28700 (131997)	131997..132197	+	201	WP_015059022.1	hypothetical protein	-
LZ608_RS28705 (132583)	132583..133182	+	600	WP_032083981.1	PIN domain-containing protein	-
LZ608_RS28710 (133244)	133244..133576	+	333	WP_152916585.1	hypothetical protein	-
- (133777)	133777..133836	-	60	NuclAT_1	-	Antitoxin
- (133777)	133777..133836	-	60	NuclAT_1	-	Antitoxin
- (133777)	133777..133836	-	60	NuclAT_1	-	Antitoxin
- (133777)	133777..133836	-	60	NuclAT_1	-	Antitoxin
LZ608_RS28715 (133881)	133881..134030	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LZ608_RS28720 (134314)	134314..134562	+	249	WP_000083837.1	replication regulatory protein RepA	-
LZ608_RS28725 (134677)	134677..134861	+	185	Protein_172	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..134873	134873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T230753 WP_001312851.1 NZ_CP090434:133881-134030 [Klebsiella pneumoniae subsp. pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T230753 NZ_CP125351:c2789920-2789543 [Escherichia fergusonii]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT230753 NZ_CP090434:c133836-133777 [Klebsiella pneumoniae subsp. pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin