Detailed information of TA system TA230725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13618..14275
• Replicon: plasmid unnamed
• Accession: NZ_CP090430
• Organism: Klebsiella pneumoniae strain DY1928

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: LYZ32_RS26275
• Protein ID: WP_000270043.1
• Coordinates: 13925..14275 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LYZ32_RS26270
• Protein ID: WP_000124640.1
• Coordinates: 13618..13920 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LYZ32_RS26225 (LYZ32_26225)	9243..9671	+	429	WP_000591074.1	hypothetical protein	-
LYZ32_RS26230 (LYZ32_26230)	9728..10087	+	360	WP_000422768.1	hypothetical protein	-
LYZ32_RS26235 (LYZ32_26235)	10087..10533	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
LYZ32_RS26240 (LYZ32_26240)	10530..11048	+	519	WP_000210756.1	nitrite reductase	-
LYZ32_RS26245 (LYZ32_26245)	11048..11278	+	231	WP_000972663.1	hypothetical protein	-
LYZ32_RS26250 (LYZ32_26250)	11265..12122	+	858	WP_001167032.1	hypothetical protein	-
LYZ32_RS26255 (LYZ32_26255)	12353..12880	+	528	WP_001236377.1	thermonuclease family protein	-
LYZ32_RS26260 (LYZ32_26260)	12938..13210	+	273	WP_001043047.1	HU family DNA-binding protein	-
LYZ32_RS26265 (LYZ32_26265)	13298..13591	+	294	WP_001239997.1	chromosome segregation protein ParM	-
LYZ32_RS26270 (LYZ32_26270)	13618..13920	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
LYZ32_RS26275 (LYZ32_26275)	13925..14275	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LYZ32_RS26280 (LYZ32_26280)	14438..14986	+	549	WP_001061574.1	transcriptional regulator	-
LYZ32_RS26285 (LYZ32_26285)	15327..15521	+	195	WP_000343597.1	hypothetical protein	-
LYZ32_RS26290 (LYZ32_26290)	15532..15903	+	372	WP_000516916.1	hypothetical protein	-
LYZ32_RS26295 (LYZ32_26295)	15896..16366	+	471	WP_001281821.1	hypothetical protein	-
LYZ32_RS26300 (LYZ32_26300)	16381..16716	-	336	WP_000683476.1	hypothetical protein	-
LYZ32_RS26305 (LYZ32_26305)	16813..17301	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
LYZ32_RS26310 (LYZ32_26310)	17304..17801	+	498	WP_000062185.1	hypothetical protein	-
LYZ32_RS26315 (LYZ32_26315)	18016..18720	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-1	-	1..147900	147900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T230725 WP_000270043.1 NZ_CP090430:c14275-13925 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T230725 NZ_CP125343:c5388411-5388028 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT230725 WP_000124640.1 NZ_CP090430:c13920-13618 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT230725 NZ_CP125343:c5388653-5388411 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9