Detailed information of TA system TA023070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1108101..1108933
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7J9L0
• Locus tag: LFERR_RS05665
• Protein ID: WP_009569577.1
• Coordinates: 1108101..1108607 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: B7J9L1
• Locus tag: LFERR_RS05670
• Protein ID: WP_009569578.1
• Coordinates: 1108604..1108933 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS05645	1104006..1104824	+	819	Protein_1117	UDP-glucose/GDP-mannose dehydrogenase family protein	-
LFERR_RS05650	1104805..1105776	+	972	WP_049759457.1	D-glycero-beta-D-manno-heptose-7-phosphate kinase	-
LFERR_RS05655	1105778..1106548	+	771	WP_012536502.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
LFERR_RS05660	1106541..1107383	+	843	WP_012536503.1	Hsp33 family molecular chaperone HslO	-
LFERR_RS05665	1108101..1108607	-	507	WP_009569577.1	type II toxin-antitoxin system YhaV family toxin	Toxin
LFERR_RS05670	1108604..1108933	-	330	WP_009569578.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
LFERR_RS15605	1109220..1109363	+	144	WP_009569579.1	hypothetical protein	-
LFERR_RS05675	1109390..1109656	-	267	WP_012536504.1	type I restriction-modification system subunit M N-terminal domain-containing protein	-
LFERR_RS05680	1110001..1110495	-	495	WP_009565062.1	GNAT family N-acetyltransferase	-
LFERR_RS05685	1110492..1110764	-	273	WP_012536505.1	DUF1778 domain-containing protein	-
LFERR_RS05690	1110845..1111057	-	213	WP_009562915.1	hypothetical protein	-
LFERR_RS05695	1111225..1111441	-	217	Protein_1128	hypothetical protein	-
LFERR_RS05700	1111841..1112662	-	822	WP_012536506.1	UDP-2,3-diacylglucosamine diphosphatase	-
LFERR_RS05705	1112903..1113148	+	246	WP_009568482.1	DUF2934 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-107)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 19373.17 Da
• Isoelectric Point: 9.9366

>T23070 WP_009569577.1 NC_011206:c1108607-1108101 [Acidithiobacillus ferrooxidans ATCC 53993]
MSSSKLAPLVIHGWTVFAHPLFLAHIETLAQQVEAFKKKDPVGYVKKNANKRLAAIIKLAFDAIPQDPTRPEYRQGNTLG
EDHKHWFRAKFFQQYRLFFRYHAPSKVIVLAWVNDEDTKRAYESSDDAYRVFRKMLESGRPPDDWNQLLAEACAEGQRLQ
RFAAGIAP

Nucleotide

• Length: 507 bp

>T23070 NC_011206:c1108607-1108101 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGAGCTCGAGCAAGCTCGCGCCCTTGGTCATTCATGGCTGGACGGTTTTTGCCCACCCGCTATTCCTCGCGCATATTGA
GACCTTGGCCCAGCAGGTCGAGGCCTTCAAGAAAAAGGATCCCGTCGGATACGTGAAAAAGAACGCCAATAAGCGACTGG
CGGCGATCATCAAACTGGCGTTCGACGCCATCCCGCAAGACCCGACACGGCCAGAGTACCGTCAAGGCAACACCCTCGGT
GAAGATCACAAGCACTGGTTCCGCGCCAAGTTTTTCCAGCAATACCGGCTGTTCTTCCGCTACCACGCGCCGAGCAAGGT
GATCGTGCTCGCCTGGGTCAATGACGAGGACACCAAGCGCGCCTATGAGAGCAGTGACGACGCCTATCGGGTGTTCCGAA
AGATGCTGGAAAGCGGCCGTCCTCCGGATGACTGGAACCAGTTGCTGGCCGAGGCATGCGCCGAGGGGCAGCGACTACAG
CGATTCGCTGCTGGCATTGCACCGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12107.54 Da
• Isoelectric Point: 4.3141

>AT23070 WP_009569578.1 NC_011206:c1108933-1108604 [Acidithiobacillus ferrooxidans ATCC 53993]
MAATLEVESTLTDRYQTTVPETVRRALRLGKRDKIHYTIRPGGEVVLTRAEDSEEDDPVLGQFLGFLARDIATHPERLQA
IDVSFVQRLQSLTGGIEVDLDAPLSADDE

Nucleotide

• Length: 330 bp

>AT23070 NC_011206:c1108933-1108604 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGGCTGCCACCCTCGAAGTCGAATCCACGCTGACTGACCGCTACCAAACCACGGTGCCGGAGACCGTCCGCCGGGCCCT
CCGGCTGGGCAAGCGCGACAAGATCCACTACACCATCCGCCCCGGTGGCGAGGTCGTGCTGACGCGCGCCGAGGACTCCG
AGGAAGACGATCCGGTGCTCGGTCAGTTCCTGGGCTTCCTGGCCCGCGACATCGCCACGCATCCAGAGCGCCTGCAGGCC
ATCGATGTCAGCTTCGTGCAGCGCCTCCAATCGCTGACCGGCGGCATCGAAGTCGATCTCGATGCCCCCTTGTCTGCGGA
CGATGAATGA

Structures

Toxin

• AlphaFold DB: B7J9L0

Antitoxin

• AlphaFold DB: B7J9L1