Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3681790..3682484 Replicon chromosome
Accession NZ_CP090402
Organism Shigella sp. PIB

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag LXH19_RS17500 Protein ID WP_001521903.1
Coordinates 3681790..3682188 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LXH19_RS17505 Protein ID WP_000554758.1
Coordinates 3682191..3682484 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3677620) 3677620..3677700 - 81 NuclAT_8 - -
- (3677620) 3677620..3677700 - 81 NuclAT_8 - -
- (3677620) 3677620..3677700 - 81 NuclAT_8 - -
- (3677620) 3677620..3677700 - 81 NuclAT_8 - -
LXH19_RS17470 (3676960) 3676960..3678204 - 1245 WP_000189541.1 esterase FrsA -
LXH19_RS17475 (3678296) 3678296..3678754 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LXH19_RS17480 (3679015) 3679015..3680472 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
LXH19_RS17485 (3680529) 3680529..3680881 - 353 Protein_3417 peptide chain release factor H -
LXH19_RS17490 (3680877) 3680877..3681083 - 207 Protein_3418 RtcB family protein -
LXH19_RS17495 (3681328) 3681328..3681780 - 453 WP_001059899.1 GNAT family N-acetyltransferase -
LXH19_RS17500 (3681790) 3681790..3682188 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LXH19_RS17505 (3682191) 3682191..3682484 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LXH19_RS17510 (3682536) 3682536..3683591 - 1056 WP_001226166.1 DNA polymerase IV -
LXH19_RS17515 (3683662) 3683662..3684447 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
LXH19_RS17520 (3684419) 3684419..3686131 + 1713 Protein_3424 flagellar biosynthesis protein FlhA -
LXH19_RS17525 (3686293) 3686293..3687042 - 750 WP_000093873.1 C40 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T230669 WP_001521903.1 NZ_CP090402:c3682188-3681790 [Shigella sp. PIB]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T230669 NZ_CP125292:c4111018-4110902 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT230669 WP_000554758.1 NZ_CP090402:c3682484-3682191 [Shigella sp. PIB]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT230669 NZ_CP125292:4110756-4110907 [Bacillus subtilis]
TTTCTTTAATGAGAAATGCATAAAATAAAAAGACCAGGGTGTTGGCGCACCCCGGCTCTGTACAAAAAGCTGCCCGTCAA
AGGGCTTGCTCGATGTTCAATCTGCGTAGACCTAACCCTTTAAGGTTCCTAAGCTCAAGGGAAGGTCTATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References