Detailed information of TA system TA023064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 87493..88101
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2W1K1W3
• Locus tag: LFERR_RS00450
• Protein ID: WP_012535782.1
• Coordinates: 87493..87768 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LFERR_RS00455
• Protein ID: WP_009566298.1
• Coordinates: 87784..88101 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS00410	83271..84311	+	1041	WP_012535779.1	glycosyltransferase family 4 protein	-
LFERR_RS00415	84379..85305	+	927	WP_012535780.1	glycosyltransferase family 2 protein	-
LFERR_RS00420	85310..85666	+	357	Protein_82	glycosyltransferase	-
LFERR_RS00435	86406..86627	+	222	WP_009566302.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LFERR_RS00440	86627..87040	+	414	WP_009566301.1	type II toxin-antitoxin system VapC family toxin	-
LFERR_RS00445	87037..87327	+	291	WP_009566300.1	hypothetical protein	-
LFERR_RS00450	87493..87768	+	276	WP_012535782.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LFERR_RS00455	87784..88101	+	318	WP_009566298.1	HigA family addiction module antidote protein	Antitoxin
LFERR_RS14695	88101..88241	+	141	Protein_88	HNH endonuclease	-
LFERR_RS00465	88715..89878	+	1164	WP_009566294.1	Fic family protein	-
LFERR_RS00470	90011..90438	-	428	Protein_90	type II toxin-antitoxin system VapC family toxin	-
LFERR_RS00475	90438..90680	-	243	WP_012535785.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LFERR_RS00480	90764..90937	-	174	WP_009562770.1	PCP reductase family protein	-
LFERR_RS00485	91048..91476	-	429	WP_009562768.1	hypothetical protein	-
LFERR_RS00490	91479..91910	-	432	WP_009562767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-91)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-69)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10433.84 Da
• Isoelectric Point: 7.9070

>T23064 WP_012535782.1 NC_011206:87493-87768 [Acidithiobacillus ferrooxidans ATCC 53993]
MIRSFVHKGLKQVFETGQSAKVGADLVTRCKRRLDAINAATSPEELNVPGFDFHALEGKPKRYTVHVNGPYCITFEWREG
DAWRIDLENYH

Nucleotide

• Length: 276 bp

>T23064 NC_011206:87493-87768 [Acidithiobacillus ferrooxidans ATCC 53993]
TTGATACGAAGTTTTGTGCATAAGGGCCTCAAGCAGGTTTTCGAAACAGGTCAATCGGCGAAAGTCGGTGCGGATTTGGT
AACCCGCTGCAAGCGCAGGCTCGATGCCATAAACGCTGCGACATCCCCGGAGGAGTTGAACGTGCCGGGTTTCGACTTCC
ATGCTTTGGAGGGGAAACCGAAGCGGTACACGGTTCATGTGAATGGACCCTATTGCATCACCTTCGAGTGGCGTGAAGGA
GATGCCTGGCGCATTGATCTGGAGAATTACCATTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11756.54 Da
• Isoelectric Point: 5.6631

>AT23064 WP_009566298.1 NC_011206:87784-88101 [Acidithiobacillus ferrooxidans ATCC 53993]
MDEMTVEQVDRIPTHPGEILREEVLPALDISVAEFARGLRVSRQTVHGILSERKGITPEMALRIGKYLGNGPGIWLRMQQ
AYDLTVAERRLAEELAAIHKPEQAA

Nucleotide

• Length: 318 bp

>AT23064 NC_011206:87784-88101 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGGACGAAATGACCGTTGAGCAAGTGGATCGGATACCGACGCATCCTGGCGAGATATTGCGGGAAGAGGTACTGCCCGC
ACTGGATATCTCTGTAGCAGAGTTTGCCCGTGGCCTGCGGGTCTCGCGCCAGACCGTTCACGGCATCCTGTCTGAGCGCA
AGGGCATCACCCCGGAGATGGCCTTGCGTATCGGCAAATATCTGGGTAACGGGCCGGGGATATGGCTACGCATGCAGCAG
GCTTACGATTTGACCGTTGCAGAACGTCGGTTGGCCGAAGAACTGGCGGCCATTCACAAGCCCGAGCAGGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2W1K1W3