Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 330055..330641 Replicon chromosome
Accession NZ_CP090398
Organism Klebsiella pneumoniae strain F94

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LZG48_RS01540 Protein ID WP_002920800.1
Coordinates 330273..330641 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag LZG48_RS01535 Protein ID WP_002920802.1
Coordinates 330055..330276 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG48_RS01515 (LZG48_01515) 326212..327138 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
LZG48_RS01520 (LZG48_01520) 327135..328412 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
LZG48_RS01525 (LZG48_01525) 328409..329176 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LZG48_RS01530 (LZG48_01530) 329178..329891 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LZG48_RS01535 (LZG48_01535) 330055..330276 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LZG48_RS01540 (LZG48_01540) 330273..330641 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LZG48_RS01545 (LZG48_01545) 330914..332230 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LZG48_RS01550 (LZG48_01550) 332337..333224 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LZG48_RS01555 (LZG48_01555) 333221..334066 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LZG48_RS01560 (LZG48_01560) 334068..335138 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 327135..335875 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T230615 WP_002920800.1 NZ_CP090398:330273-330641 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T230615 NZ_CP125092:1334-1648 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGAGATTTACAACGCCTCCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGCCGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CATTTCTGCCGCTGGAATATCAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCGGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT230615 WP_002920802.1 NZ_CP090398:330055-330276 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT230615 NZ_CP125092:1068-1346 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AAGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCAGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References