Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3845839..3846436 Replicon chromosome
Accession NZ_CP090397
Organism Klebsiella pneumoniae strain F4

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LZG49_RS18665 Protein ID WP_004142563.1
Coordinates 3846119..3846436 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LZG49_RS18660 Protein ID WP_004142561.1
Coordinates 3845839..3846126 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG49_RS18630 (3841919) 3841919..3842167 + 249 WP_020325243.1 DUF1158 domain-containing protein -
LZG49_RS18635 (3842185) 3842185..3842526 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LZG49_RS18640 (3842557) 3842557..3843672 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
LZG49_RS18645 (3843852) 3843852..3844433 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
LZG49_RS18650 (3844433) 3844433..3844801 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
LZG49_RS18655 (3844921) 3844921..3845574 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LZG49_RS18660 (3845839) 3845839..3846126 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LZG49_RS18665 (3846119) 3846119..3846436 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LZG49_RS18670 (3846621) 3846621..3847664 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
LZG49_RS18675 (3848334) 3848334..3849200 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LZG49_RS18680 (3849309) 3849309..3850736 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T230609 WP_004142563.1 NZ_CP090397:c3846436-3846119 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T230609 NZ_CP125088:c4681582-4681049 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT230609 WP_004142561.1 NZ_CP090397:c3846126-3845839 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT230609 NZ_CP125088:c4681859-4681593 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References