Detailed information of TA system TA230587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3851669..3852266
• Replicon: chromosome
• Accession: NZ_CP090393
• Organism: Klebsiella pneumoniae strain YZH2429

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LY283_RS19030
• Protein ID: WP_004893639.1
• Coordinates: 3851949..3852266 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LY283_RS19025
• Protein ID: WP_004142561.1
• Coordinates: 3851669..3851956 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY283_RS18995 (3847887)	3847887..3848135	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
LY283_RS19000 (3848153)	3848153..3848494	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LY283_RS19005 (3848525)	3848525..3849640	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
LY283_RS19010 (3849820)	3849820..3850390	+	571	Protein_3729	TetR/AcrR family transcriptional regulator	-
LY283_RS19015 (3850390)	3850390..3850758	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LY283_RS19020 (3850878)	3850878..3851531	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LY283_RS19025 (3851669)	3851669..3851956	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LY283_RS19030 (3851949)	3851949..3852266	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LY283_RS19035 (3852451)	3852451..3853494	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
LY283_RS19040 (3854164)	3854164..3855030	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LY283_RS19045 (3855139)	3855139..3856566	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T230587 WP_004893639.1 NZ_CP090393:c3852266-3851949 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T230587 NZ_CP125083:4743382-4743867 [Klebsiella pneumoniae subsp. pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGATAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT230587 WP_004142561.1 NZ_CP090393:c3851956-3851669 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT230587 NZ_CP125083:4743092-4743385 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1