Detailed information of TA system TA230565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3851666..3852263
• Replicon: chromosome
• Accession: NZ_CP090389
• Organism: Klebsiella pneumoniae strain YZH22127

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LZC40_RS19030
• Protein ID: WP_004893639.1
• Coordinates: 3851946..3852263 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LZC40_RS19025
• Protein ID: WP_004142561.1
• Coordinates: 3851666..3851953 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZC40_RS18995 (3847884)	3847884..3848132	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
LZC40_RS19000 (3848150)	3848150..3848491	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LZC40_RS19005 (3848522)	3848522..3849637	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
LZC40_RS19010 (3849817)	3849817..3850387	+	571	Protein_3729	TetR/AcrR family transcriptional regulator	-
LZC40_RS19015 (3850387)	3850387..3850755	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LZC40_RS19020 (3850875)	3850875..3851528	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LZC40_RS19025 (3851666)	3851666..3851953	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LZC40_RS19030 (3851946)	3851946..3852263	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LZC40_RS19035 (3852448)	3852448..3853491	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
LZC40_RS19040 (3854161)	3854161..3855027	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LZC40_RS19045 (3855136)	3855136..3856563	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T230565 WP_004893639.1 NZ_CP090389:c3852263-3851946 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T230565 NZ_CP124965:112840-113133 [Agrobacterium fabacearum]
ATGCCATTCAGCCTATCGGTCCAAGCGGAAGAGGATATTATTTTCATTGCAGAAGAAGGCATCCGCATCTTCGGAGCACT
AGTCGCCAAACGATATCACGATGAGCTTTTTGCTTTGCTCGAGCTCATCGCCACCAACCCAAGAATGGCACGCGAACGCC
ATGAGATTTCTCCTCCTGTCAGGATACATCCTTTCAAAGCTCACCTTGTCGTGTATCGCATCATTGAAGACGGGAGCGTA
TTCGTCATCCGCATACGGCACGGCCATGAAGATTGGGCGGGAGATTCTTTCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT230565 WP_004142561.1 NZ_CP090389:c3851953-3851666 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT230565 NZ_CP124965:112586-112840 [Agrobacterium fabacearum]
ATGGCGACGATGAATGTATCTCTGCCAGACCCCATGAAAGATTGGGTTGAAGCACAGGCCAAGACAGGACGATACTCGAA
TGCAAGCGATTATGTCCGGGACCTTATCCGCAGAGACCAAGCCCGTACGGATAAGATTGCTGAGATGCAGCGATTTGTCG
ACGACGGGATCAGAAGCGGAGTTGGGAGCCGTTCAACGGAAGAGTTGTTTGCTTTAGCCACCTCTCGAGCCGCCATCAGC
CGCGGACGCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1