Detailed information of TA system TA230501

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1548178..1548788
• Replicon: chromosome
• Accession: NZ_CP090366
• Organism: Stutzerimonas kunmingensis strain 7850S

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A023WUP6
• Locus tag: LW136_RS07210
• Protein ID: WP_038662341.1
• Coordinates: 1548178..1548492 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: I4CQU1
• Locus tag: LW136_RS07215
• Protein ID: WP_003302338.1
• Coordinates: 1548498..1548788 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW136_RS07190 (LW136_07190)	1544183..1544509	+	327	WP_042926027.1	DUF2523 domain-containing protein	-
LW136_RS07195 (LW136_07195)	1544522..1545700	+	1179	WP_234174819.1	zonular occludens toxin domain-containing protein	-
LW136_RS07200 (LW136_07200)	1545848..1547126	+	1279	Protein_1416	hypothetical protein	-
LW136_RS07205 (LW136_07205)	1547126..1548133	+	1008	WP_234174820.1	tyrosine-type recombinase/integrase	-
LW136_RS07210 (LW136_07210)	1548178..1548492	+	315	WP_038662341.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LW136_RS07215 (LW136_07215)	1548498..1548788	+	291	WP_003302338.1	helix-turn-helix domain-containing protein	Antitoxin
LW136_RS07220 (LW136_07220)	1548851..1549504	+	654	WP_234174821.1	hypothetical protein	-
LW136_RS07225 (LW136_07225)	1549891..1551003	+	1113	WP_234174822.1	integrase family protein	-
LW136_RS07230 (LW136_07230)	1551007..1551207	-	201	WP_181092064.1	AlpA family phage regulatory protein	-
LW136_RS07235 (LW136_07235)	1551194..1551505	-	312	WP_213574678.1	hypothetical protein	-
LW136_RS07240 (LW136_07240)	1551516..1552019	-	504	WP_234174823.1	hypothetical protein	-
LW136_RS07245 (LW136_07245)	1552079..1552915	-	837	WP_234174824.1	DUF2303 family protein	-
LW136_RS07250 (LW136_07250)	1552954..1553301	-	348	WP_234174825.1	hypothetical protein	-
LW136_RS07255 (LW136_07255)	1553326..1553589	-	264	WP_038662318.1	pyocin activator PrtN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1534544..1552299	17755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11923.68 Da
• Isoelectric Point: 9.8035

>T230501 WP_038662341.1 NZ_CP090366:1548178-1548492 [Stutzerimonas kunmingensis]
MIFIETPIFSKRLRELLSDDSYAEFQRQLADRPDMGDVIEGTGGIRKVRVASGGHGKRGGSRVIYYHFTAASQIALLLIY
PKNEKDDLTADERKVLKQIIERWR

Nucleotide

• Length: 315 bp

>T230501 NZ_CP124912:c45159-44884 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10925.51 Da
• Isoelectric Point: 8.8068

>AT230501 WP_003302338.1 NZ_CP090366:1548498-1548788 [Stutzerimonas kunmingensis]
MSKFFEDLLESVQQMDEIHRGERQPSREFVVDSLQVKEIRKATGLTQAKFAAMIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVIQALSSV

Nucleotide

• Length: 291 bp

>AT230501 NZ_CP124912:c45418-45152 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A023WUP6

Antitoxin

• AlphaFold DB: I4CQU1