Detailed information of TA system TA230472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5352610..5353205
• Replicon: chromosome
• Accession: NZ_CP090348
• Organism: Pseudomonas aeruginosa strain PA8329

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LXN03_RS24925
• Protein ID: WP_071534808.1
• Coordinates: 5352927..5353205 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LXN03_RS24920
• Protein ID: WP_004352673.1
• Coordinates: 5352610..5352915 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN03_RS24895 (LXN03_24895)	5347630..5348064	-	435	WP_004352681.1	hypothetical protein	-
LXN03_RS24900 (LXN03_24900)	5348603..5348914	-	312	WP_180719138.1	DUF5447 family protein	-
LXN03_RS24905 (LXN03_24905)	5348980..5349252	-	273	WP_176450372.1	DNA-binding protein	-
LXN03_RS24910 (LXN03_24910)	5349364..5349636	+	273	WP_180719132.1	DNA-binding protein	-
LXN03_RS24915 (LXN03_24915)	5349695..5352265	+	2571	WP_217953707.1	hypothetical protein	-
LXN03_RS24920 (LXN03_24920)	5352610..5352915	-	306	WP_004352673.1	HigA family addiction module antitoxin	Antitoxin
LXN03_RS24925 (LXN03_24925)	5352927..5353205	-	279	WP_071534808.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LXN03_RS24930 (LXN03_24930)	5353258..5353386	-	129	Protein_4931	integrase	-
LXN03_RS24935 (LXN03_24935)	5353534..5355762	+	2229	WP_004352671.1	TonB-dependent receptor	-
LXN03_RS24940 (LXN03_24940)	5355832..5356479	-	648	WP_003095021.1	carbonate dehydratase	-
LXN03_RS24945 (LXN03_24945)	5356541..5357779	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10671.25 Da
• Isoelectric Point: 8.5576

>T230472 WP_071534808.1 NZ_CP090348:c5353205-5352927 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHGIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T230472 NZ_CP124906:c2560446-2560303 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11238.80 Da
• Isoelectric Point: 4.7769

>AT230472 WP_004352673.1 NZ_CP090348:c5352915-5352610 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT230472 NZ_CP124906:2560183-2560370 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGCAGAGCCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGT
GCAATCAAAGCAATGGTAAACATACCAA