Detailed information of TA system TA230461

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 75219..75645
• Replicon: plasmid pKPK3-2
• Accession: NZ_CP090345
• Organism: Klebsiella pneumoniae strain KPK3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LXL84_RS28230
• Protein ID: WP_001372321.1
• Coordinates: 75219..75344 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	75421..75645 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXL84_RS28195 (70666)	70666..70989	+	324	Protein_79	class II aldolase/adducin family protein	-
LXL84_RS28200 (71043)	71043..71111	-	69	Protein_80	hypothetical protein	-
LXL84_RS28205 (71472)	71472..71855	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LXL84_RS28210 (72132)	72132..72779	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LXL84_RS28215 (73076)	73076..73897	-	822	WP_001234445.1	DUF932 domain-containing protein	-
LXL84_RS28220 (74008)	74008..74304	-	297	WP_001272251.1	hypothetical protein	-
LXL84_RS28225 (74604)	74604..74900	+	297	Protein_85	hypothetical protein	-
LXL84_RS28230 (75219)	75219..75344	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LXL84_RS28235 (75286)	75286..75435	-	150	Protein_87	plasmid maintenance protein Mok	-
- (75421)	75421..75645	-	225	NuclAT_0	-	Antitoxin
- (75421)	75421..75645	-	225	NuclAT_0	-	Antitoxin
- (75421)	75421..75645	-	225	NuclAT_0	-	Antitoxin
- (75421)	75421..75645	-	225	NuclAT_0	-	Antitoxin
LXL84_RS28240 (75657)	75657..76376	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
LXL84_RS28245 (76373)	76373..76807	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LXL84_RS28250 (76876)	76876..78899	-	2024	Protein_90	ParB/RepB/Spo0J family partition protein	-
LXL84_RS28255 (78960)	78960..79193	-	234	WP_000006003.1	DUF905 family protein	-
LXL84_RS28260 (79251)	79251..79778	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
LXL84_RS28265 (80080)	80080..80535	+	456	Protein_93	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-187 / rmtB / blaTEM-1B / blaCTX-M-65	-	1..132507	132507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T230461 WP_001372321.1 NZ_CP090345:c75344-75219 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T230461 NZ_CP124905:c256508-256365 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT230461 NZ_CP090345:c75645-75421 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin