Detailed information of TA system TA230459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 88730..89457
• Replicon: plasmid pKPK3-1
• Accession: NZ_CP090344
• Organism: Klebsiella pneumoniae strain KPK3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LXL84_RS27270
• Protein ID: WP_011251285.1
• Coordinates: 89146..89457 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LXL84_RS27265
• Protein ID: WP_011251286.1
• Coordinates: 88730..89149 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXL84_RS27235 (LXL84_27200)	83898..84368	-	471	WP_048333570.1	hypothetical protein	-
LXL84_RS27240 (LXL84_27205)	84681..85316	-	636	WP_223171879.1	hypothetical protein	-
LXL84_RS27245 (LXL84_27210)	85735..86355	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LXL84_RS27250 (LXL84_27215)	86376..87164	+	789	WP_040217257.1	hypothetical protein	-
LXL84_RS27255 (LXL84_27220)	87178..87543	+	366	WP_048333448.1	hypothetical protein	-
LXL84_RS27260 (LXL84_27225)	87615..88583	-	969	WP_074428168.1	IS5 family transposase	-
LXL84_RS27265 (LXL84_27230)	88730..89149	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LXL84_RS27270 (LXL84_27235)	89146..89457	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LXL84_RS27275 (LXL84_27240)	89662..90099	-	438	Protein_106	DDE-type integrase/transposase/recombinase	-
LXL84_RS27280 (LXL84_27245)	90234..90931	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LXL84_RS27285 (LXL84_27250)	92317..92967	-	651	WP_068893702.1	DUF1173 family protein	-
LXL84_RS27290 (LXL84_27255)	93000..93266	-	267	WP_223175074.1	DUF1173 family protein	-
LXL84_RS27295 (LXL84_27260)	93444..93938	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN	1..189486	189486
	-	inside	IScluster/Tn	-	-	80890..105264	24374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T230459 WP_011251285.1 NZ_CP090344:c89457-89146 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T230459 NZ_CP124901:2740830-2740973 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT230459 WP_011251286.1 NZ_CP090344:c89149-88730 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT230459 NZ_CP124901:c2741167-2740905 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGACAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1