Detailed information of TA system TA230419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4936845..4937539
• Replicon: chromosome
• Accession: NZ_CP090311
• Organism: Pseudomonas kielensis strain 5BT

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LZV00_RS23010
• Protein ID: WP_182343206.1
• Coordinates: 4936845..4937141 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LZV00_RS23015
• Protein ID: WP_103397313.1
• Coordinates: 4937138..4937539 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZV00_RS22975 (LZV00_22930)	4932524..4933069	-	546	WP_265537088.1	hypothetical protein	-
LZV00_RS22980 (LZV00_22935)	4933066..4933320	-	255	WP_182343202.1	acyl carrier protein	-
LZV00_RS22985 (LZV00_22940)	4933330..4933590	-	261	WP_166592761.1	phosphopantetheine-binding protein	-
LZV00_RS22990 (LZV00_22945)	4933571..4934380	-	810	WP_166592760.1	lysophospholipid acyltransferase family protein	-
LZV00_RS22995 (LZV00_22950)	4934356..4935075	-	720	WP_265537089.1	beta-ketoacyl synthase chain length factor	-
LZV00_RS23000 (LZV00_22955)	4935431..4936201	+	771	WP_166592758.1	ParA family protein	-
LZV00_RS23005 (LZV00_22960)	4936216..4936650	-	435	WP_166592757.1	thioredoxin TrxC	-
LZV00_RS23010 (LZV00_22965)	4936845..4937141	+	297	WP_182343206.1	type II toxin-antitoxin system HigB family toxin	Toxin
LZV00_RS23015 (LZV00_22970)	4937138..4937539	+	402	WP_103397313.1	transcriptional regulator	Antitoxin
LZV00_RS23020 (LZV00_22975)	4937546..4939009	-	1464	WP_265537091.1	YdiU family protein	-
LZV00_RS23025 (LZV00_22980)	4939058..4942408	-	3351	WP_182343210.1	mechanosensitive channel MscK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4936845..4956236	19391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (19-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (78-132)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11482.36 Da
• Isoelectric Point: 10.5487

>T230419 WP_182343206.1 NZ_CP090311:4936845-4937141 [Pseudomonas kielensis]
MHVLTEKRVREAKVRWPMAANALDHWYQLAKGSKPSDFAAMRATFPAVDKVGELHVFDIGGNKLRLIAFVRYRSQKLYIK
PVLDHREYDRGKWKEEKA

Nucleotide

• Length: 297 bp

>T230419 NZ_CP124893:c2789101-2788760 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 15178.37 Da
• Isoelectric Point: 4.8298

>AT230419 WP_103397313.1 NZ_CP090311:4937138-4937539 [Pseudomonas kielensis]
MSVLIKKAAEHWEFVSPLLRKPKSEDDYDLLAHALDELLEMTGDDESHPLMSLVDIIGDWIEEWDHKHHPMPKPSGVEIL
GYLMREHGLSQSDLPGVGPQSVVSEILSGKRQLNLRQVRWLAERFSVSVEVFI

Nucleotide

• Length: 402 bp

>AT230419 NZ_CP124893:c2789331-2789101 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA