Detailed information of TA system TA230416

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44183..44452
• Replicon: plasmid p503-IncFII
• Accession: NZ_CP090306
• Organism: Escherichia coli strain Z503

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LXF06_RS21825
• Protein ID: WP_001372321.1
• Coordinates: 44327..44452 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	44183..44248 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXF06_RS21790	39893..40420	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LXF06_RS21795	40478..40711	+	234	WP_000006003.1	DUF905 family protein	-
LXF06_RS21800	40772..42795	+	2024	Protein_51	ParB/RepB/Spo0J family partition protein	-
LXF06_RS21805	42864..43298	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LXF06_RS21810	43295..44057	+	763	Protein_53	plasmid SOS inhibition protein A	-
-	44026..44250	+	225	NuclAT_0	-	-
-	44026..44250	+	225	NuclAT_0	-	-
-	44026..44250	+	225	NuclAT_0	-	-
-	44026..44250	+	225	NuclAT_0	-	-
LXF06_RS21815	44035..44214	-	180	WP_001309233.1	hypothetical protein	-
-	44183..44248	-	66	-	-	Antitoxin
LXF06_RS21820	44236..44385	+	150	Protein_55	plasmid maintenance protein Mok	-
LXF06_RS21825	44327..44452	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LXF06_RS21830	44771..45067	-	297	Protein_57	hypothetical protein	-
LXF06_RS21835	45367..45663	+	297	WP_001272251.1	hypothetical protein	-
LXF06_RS21840	45774..46595	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LXF06_RS21845	46892..47482	-	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
LXF06_RS21850	47817..48200	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LXF06_RS21855	48394..49065	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
LXF06_RS21860	49202..49429	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T230416 WP_001372321.1 NZ_CP090306:44327-44452 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T230416 NZ_CP124893:619578-619694 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT230416 NZ_CP090306:c44248-44183 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin