Detailed information of TA system TA230396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 9401..10095
• Replicon: chromosome
• Accession: NZ_CP090305
• Organism: Escherichia coli strain Z503

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: LXF06_RS00060
• Protein ID: WP_001263493.1
• Coordinates: 9401..9799 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: LXF06_RS00065
• Protein ID: WP_000554757.1
• Coordinates: 9802..10095 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (5061)	5061..5141	-	81	NuclAT_9	-	-
- (5061)	5061..5141	-	81	NuclAT_9	-	-
- (5061)	5061..5141	-	81	NuclAT_9	-	-
- (5061)	5061..5141	-	81	NuclAT_9	-	-
LXF06_RS00030 (4401)	4401..5645	-	1245	WP_000189541.1	esterase FrsA	-
LXF06_RS00035 (5737)	5737..6195	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
LXF06_RS00040 (6456)	6456..7913	+	1458	WP_088474887.1	cytosol nonspecific dipeptidase	-
LXF06_RS00045 (7970)	7970..8491	-	522	Protein_7	peptide chain release factor H	-
LXF06_RS00050 (8490)	8490..8693	-	204	Protein_8	RtcB family protein	-
LXF06_RS00055 (8939)	8939..9391	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
LXF06_RS00060 (9401)	9401..9799	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LXF06_RS00065 (9802)	9802..10095	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LXF06_RS00070 (10147)	10147..11202	-	1056	WP_001226164.1	DNA polymerase IV	-
LXF06_RS00075 (11273)	11273..12196	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
LXF06_RS00080 (12199)	12199..13062	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
LXF06_RS00085 (13075)	13075..13680	-	606	Protein_15	FliA/WhiG family RNA polymerase sigma factor	-
LXF06_RS00090 (13736)	13736..14433	+	698	WP_241415623.1	IS1 family transposase	-
LXF06_RS00095 (14541)	14541..14912	+	372	WP_001752077.1	FliM/FliN family flagellar motor switch protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T230396 WP_001263493.1 NZ_CP090305:c9799-9401 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T230396 NZ_CP124889:c276243-276100 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT230396 WP_000554757.1 NZ_CP090305:c10095-9802 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT230396 NZ_CP124889:275983-276167 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6