Detailed information of TA system TA230340

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 181664..182391
• Replicon: plasmid pKPNSF-2-1
• Accession: NZ_CP090243
• Organism: Klebsiella pneumoniae strain KPNSF-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LXH14_RS27225
• Protein ID: WP_011251285.1
• Coordinates: 181664..181975 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LXH14_RS27230
• Protein ID: WP_011251286.1
• Coordinates: 181972..182391 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH14_RS27200 (LXH14_27200)	177182..177676	-	495	WP_004212794.1	thermonuclease family protein	-
LXH14_RS27205 (LXH14_27205)	177855..178121	+	267	WP_223175074.1	DUF1173 family protein	-
LXH14_RS27210 (LXH14_27210)	178154..178804	+	651	WP_068893702.1	DUF1173 family protein	-
LXH14_RS27220 (LXH14_27220)	181022..181459	+	438	Protein_204	DDE-type integrase/transposase/recombinase	-
LXH14_RS27225 (LXH14_27225)	181664..181975	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LXH14_RS27230 (LXH14_27230)	181972..182391	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LXH14_RS27235 (LXH14_27235)	182538..183506	+	969	WP_074428168.1	IS5 family transposase	-
LXH14_RS27240 (LXH14_27240)	183578..183943	-	366	WP_048333448.1	hypothetical protein	-
LXH14_RS27245 (LXH14_27245)	183957..184745	-	789	WP_040217257.1	hypothetical protein	-
LXH14_RS27250 (LXH14_27250)	184766..185386	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LXH14_RS27255 (LXH14_27255)	185805..186437	+	633	WP_233999467.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..226910	226910
	-	inside	IScluster/Tn	-	-	180190..191706	11516

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T230340 WP_011251285.1 NZ_CP090243:181664-181975 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T230340 NZ_CP124873:c2643366-2642044 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGATAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCACTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGTTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGCGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCTATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGACTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT230340 WP_011251286.1 NZ_CP090243:181972-182391 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT230340 NZ_CP124873:c2643632-2643366 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1