Detailed information of TA system TA230302

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 170905..171158
• Replicon: plasmid pBY221-1
• Accession: NZ_CP090219
• Organism: Klebsiella pneumoniae strain BY221

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LVJ43_RS26525
• Protein ID: WP_001312851.1
• Coordinates: 171009..171158 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	170905..170964 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ43_RS26485 (166565)	166565..166630	-	66	Protein_217	helix-turn-helix domain-containing protein	-
LVJ43_RS26490 (166683)	166683..167387	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LVJ43_RS26495 (167412)	167412..167612	+	201	WP_072354025.1	hypothetical protein	-
LVJ43_RS26500 (167632)	167632..168378	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LVJ43_RS26505 (168433)	168433..168993	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LVJ43_RS26510 (169125)	169125..169325	+	201	WP_015059022.1	hypothetical protein	-
LVJ43_RS26515 (169711)	169711..170310	+	600	WP_032083981.1	PIN domain-containing protein	-
LVJ43_RS26520 (170372)	170372..170704	+	333	WP_152916585.1	hypothetical protein	-
- (170905)	170905..170964	-	60	NuclAT_1	-	Antitoxin
- (170905)	170905..170964	-	60	NuclAT_1	-	Antitoxin
- (170905)	170905..170964	-	60	NuclAT_1	-	Antitoxin
- (170905)	170905..170964	-	60	NuclAT_1	-	Antitoxin
LVJ43_RS26525 (171009)	171009..171158	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LVJ43_RS26530 (171442)	171442..171690	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2	-	1..172001	172001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T230302 WP_001312851.1 NZ_CP090219:171009-171158 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T230302 NZ_CP124831:559371-559721 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT230302 NZ_CP090219:c170964-170905 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin