Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 22486..23213 | Replicon | plasmid pJNKPN26_Vir |
| Accession | NZ_CP090206 | ||
| Organism | Klebsiella pneumoniae strain JNKPN26 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | LXM99_RS28875 | Protein ID | WP_011251285.1 |
| Coordinates | 22902..23213 (-) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LXM99_RS28870 | Protein ID | WP_011251286.1 |
| Coordinates | 22486..22905 (-) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LXM99_RS28840 (LXM99_28840) | 17654..18124 | - | 471 | WP_048333570.1 | hypothetical protein | - |
| LXM99_RS28845 (LXM99_28845) | 18437..19072 | - | 636 | WP_223171879.1 | hypothetical protein | - |
| LXM99_RS28850 (LXM99_28850) | 19491..20111 | + | 621 | WP_223175004.1 | conjugative transfer protein MobI(A/C) | - |
| LXM99_RS28855 (LXM99_28855) | 20132..20920 | + | 789 | WP_040217257.1 | hypothetical protein | - |
| LXM99_RS28860 (LXM99_28860) | 20934..21299 | + | 366 | WP_048333448.1 | hypothetical protein | - |
| LXM99_RS28865 (LXM99_28865) | 21371..22339 | - | 969 | WP_074428168.1 | IS5 family transposase | - |
| LXM99_RS28870 (LXM99_28870) | 22486..22905 | - | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| LXM99_RS28875 (LXM99_28875) | 22902..23213 | - | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| LXM99_RS28880 (LXM99_28880) | 23418..23855 | - | 438 | Protein_24 | DDE-type integrase/transposase/recombinase | - |
| LXM99_RS28885 (LXM99_28885) | 23990..24687 | + | 698 | WP_223175001.1 | IS1-like element IS1A family transposase | - |
| LXM99_RS28890 (LXM99_28890) | 24689..25138 | + | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| LXM99_RS28895 (LXM99_28895) | 25155..25466 | + | 312 | WP_011251282.1 | hypothetical protein | - |
| LXM99_RS28900 (LXM99_28900) | 25480..25821 | + | 342 | WP_011251281.1 | hypothetical protein | - |
| LXM99_RS28905 (LXM99_28905) | 25881..26837 | + | 957 | WP_011251280.1 | DsbA family protein | - |
| LXM99_RS28910 (LXM99_28910) | 27262..27702 | - | 441 | WP_011251275.1 | hypothetical protein | - |
| LXM99_RS28915 (LXM99_28915) | 27708..28202 | - | 495 | WP_011251274.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..220975 | 220975 | |
| - | inside | IScluster/Tn | - | - | 5815..30122 | 24307 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T230242 WP_011251285.1 NZ_CP090206:c23213-22902 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T230242 NZ_CP124813:c322131-321814 [Citrobacter freundii]
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA
ATGTTCACATTTATTGAGCTACAAGGATTTAGTAAACGCCGTCCGTTACTTTTGCCTGACGATGAGTTTCGCGCTTTTCA
AGAGGCGCTTATAGAGAACCCTGAGGCTGGAGATACTATCGCTGGTACAGGTGGATTCAGAAAAATTCGCTGGAGTCGCT
CTGGTATGGGCAAACGCAGCGGTATTAGGGTTATCTATTACAACGTGACGCGCAAAGGTCGAATCTATCTCGCTTTGCTC
TATCCTAAAAATGAACAGGATGATTTAACCGAAGAGCAAAAAAGAGTTTTAATGCACTTATCCAATATGCTGATTTAA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT230242 WP_011251286.1 NZ_CP090206:c22905-22486 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT230242 NZ_CP124813:c321761-321465 [Citrobacter freundii]
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA
ATGAAAGACGAATTATTTGCCGATTTGCTGGCCAGTGCAGAAGAAATGGTGCGTATAGAGAAAGGTGAAGAAACACCTAA
GCCTGAACATGTACATACCTTTAGCGAAATTGATGTTAAAGCCATCCGCGAAGCAACAGGGTTACGCCAACAGGATTTTG
CCATAGCTGTTGGCGTGAGTTATGACCTGGTTAAAAGCTGGGAAACGAAACGACGCCAGCCGACTGGTGCACCAAGAAAA
CTGCTTTTATTAATTCAAAAAAATCCATTTATAATTAATCAATTAAAAGCAATTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|