Detailed information of TA system TA230236

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53524..53777
• Replicon: plasmid pJNKPN26_KPC
• Accession: NZ_CP090204
• Organism: Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LXM99_RS27420
• Protein ID: WP_001312851.1
• Coordinates: 53524..53673 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	53718..53777 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM99_RS27380 (48883)	48883..49298	-	416	Protein_67	IS1-like element IS1B family transposase	-
LXM99_RS27385 (49547)	49547..49948	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LXM99_RS27390 (49881)	49881..50138	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LXM99_RS27395 (50231)	50231..50884	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LXM99_RS27400 (51823)	51823..52680	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
LXM99_RS27410 (52699)	52699..52877	-	179	Protein_72	protein CopA/IncA	-
LXM99_RS27415 (52992)	52992..53240	-	249	WP_000083837.1	replication regulatory protein RepA	-
LXM99_RS27420 (53524)	53524..53673	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (53718)	53718..53777	+	60	NuclAT_1	-	Antitoxin
- (53718)	53718..53777	+	60	NuclAT_1	-	Antitoxin
- (53718)	53718..53777	+	60	NuclAT_1	-	Antitoxin
- (53718)	53718..53777	+	60	NuclAT_1	-	Antitoxin
LXM99_RS27425 (53978)	53978..54310	-	333	WP_152916585.1	hypothetical protein	-
LXM99_RS27430 (54372)	54372..54971	-	600	WP_032083981.1	PIN domain-containing protein	-
LXM99_RS27435 (55357)	55357..55557	-	201	WP_015059022.1	hypothetical protein	-
LXM99_RS27440 (55689)	55689..56249	-	561	WP_000139328.1	fertility inhibition protein FinO	-
LXM99_RS27445 (56304)	56304..57050	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LXM99_RS27450 (57070)	57070..57270	-	201	WP_072354025.1	hypothetical protein	-
LXM99_RS27455 (57295)	57295..57999	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LXM99_RS27460 (58054)	58054..58419	-	366	Protein_82	type IV conjugative transfer system protein TraE	-
LXM99_RS27465 (58439)	58439..58744	-	306	WP_004144424.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..126203	126203
	-	flank	IS/Tn	-	-	57295..57999	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T230236 WP_001312851.1 NZ_CP090204:c53673-53524 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T230236 NZ_CP124809:c4133267-4132860 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCTGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAGC
AGAAGTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT230236 NZ_CP090204:53718-53777 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin