Detailed information of TA system TA230233

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 17363..17789
• Replicon: plasmid pJNKPN26_KPC
• Accession: NZ_CP090204
• Organism: Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LXM99_RS27170
• Protein ID: WP_001372321.1
• Coordinates: 17363..17488 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	17565..17789 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM99_RS27135 (12418)	12418..12645	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
LXM99_RS27140 (12739)	12739..13425	-	687	WP_015059009.1	PAS domain-containing protein	-
LXM99_RS27145 (13616)	13616..13999	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LXM99_RS27150 (14276)	14276..14923	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LXM99_RS27155 (15220)	15220..16041	-	822	WP_001234445.1	DUF932 domain-containing protein	-
LXM99_RS27160 (16152)	16152..16448	-	297	WP_001272251.1	hypothetical protein	-
LXM99_RS27165 (16748)	16748..17044	+	297	Protein_24	hypothetical protein	-
LXM99_RS27170 (17363)	17363..17488	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LXM99_RS27175 (17430)	17430..17579	-	150	Protein_26	plasmid maintenance protein Mok	-
- (17565)	17565..17789	-	225	NuclAT_0	-	Antitoxin
- (17565)	17565..17789	-	225	NuclAT_0	-	Antitoxin
- (17565)	17565..17789	-	225	NuclAT_0	-	Antitoxin
- (17565)	17565..17789	-	225	NuclAT_0	-	Antitoxin
LXM99_RS27180 (17801)	17801..18520	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
LXM99_RS27185 (18517)	18517..18951	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LXM99_RS27190 (19020)	19020..21043	-	2024	Protein_29	ParB/RepB/Spo0J family partition protein	-
LXM99_RS27195 (21104)	21104..21337	-	234	WP_000006003.1	DUF905 family protein	-
LXM99_RS27200 (21395)	21395..21922	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
LXM99_RS27205 (22224)	22224..22679	+	456	Protein_32	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 / blaSHV-12	-	1..126203	126203

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T230233 WP_001372321.1 NZ_CP090204:c17488-17363 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T230233 NZ_CP124809:c1221754-1221536 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT230233 NZ_CP090204:c17789-17565 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin