Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4553483..4554129 Replicon chromosome
Accession NZ_CP090203
Organism Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag LXM99_RS22195 Protein ID WP_002920560.1
Coordinates 4553483..4553830 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LXM99_RS22200 Protein ID WP_002920557.1
Coordinates 4553830..4554129 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXM99_RS22185 (LXM99_22185) 4549409..4550842 + 1434 WP_002920564.1 glycogen synthase GlgA -
LXM99_RS22190 (LXM99_22190) 4550860..4553307 + 2448 WP_002920561.1 glycogen phosphorylase -
LXM99_RS22195 (LXM99_22195) 4553483..4553830 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LXM99_RS22200 (LXM99_22200) 4553830..4554129 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LXM99_RS22205 (LXM99_22205) 4554192..4555700 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LXM99_RS22210 (LXM99_22210) 4555905..4556234 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LXM99_RS22215 (LXM99_22215) 4556285..4557115 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
LXM99_RS22220 (LXM99_22220) 4557165..4557923 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T230230 WP_002920560.1 NZ_CP090203:4553483-4553830 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T230230 NZ_CP124809:240761-241036 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATTAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT230230 WP_002920557.1 NZ_CP090203:4553830-4554129 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT230230 NZ_CP124809:241033-241392 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References