Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4527294..4527880 Replicon chromosome
Accession NZ_CP090203
Organism Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag LXM99_RS22085 Protein ID WP_002920800.1
Coordinates 4527512..4527880 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag LXM99_RS22080 Protein ID WP_002920802.1
Coordinates 4527294..4527515 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXM99_RS22050 (LXM99_22050) 4522993..4523376 - 384 WP_002920810.1 aspartate 1-decarboxylase autocleavage activator PanM -
LXM99_RS22055 (LXM99_22055) 4523800..4524909 + 1110 WP_002920808.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
LXM99_RS22060 (LXM99_22060) 4524971..4525189 + 219 Protein_4315 branched-chain amino acid ABC transporter permease LivH -
LXM99_RS22065 (LXM99_22065) 4525181..4525651 + 471 Protein_4316 branched chain amino acid ABC transporter permease LivM -
LXM99_RS22070 (LXM99_22070) 4525648..4526415 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
LXM99_RS22075 (LXM99_22075) 4526417..4527130 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
LXM99_RS22080 (LXM99_22080) 4527294..4527515 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LXM99_RS22085 (LXM99_22085) 4527512..4527880 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LXM99_RS22090 (LXM99_22090) 4528153..4529469 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
LXM99_RS22095 (LXM99_22095) 4529576..4530463 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
LXM99_RS22100 (LXM99_22100) 4530460..4531305 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
LXM99_RS22105 (LXM99_22105) 4531307..4532377 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4525648..4533114 7466


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T230229 WP_002920800.1 NZ_CP090203:4527512-4527880 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T230229 NZ_CP124809:34610-34972 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT230229 WP_002920802.1 NZ_CP090203:4527294-4527515 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT230229 NZ_CP124809:34959-35258 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References