Detailed information of TA system TA230212

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 74381..74802
• Replicon: plasmid p1
• Accession: NZ_CP090197
• Organism: Escherichia coli strain YL3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LXN07_RS24935
• Protein ID: WP_223412041.1
• Coordinates: 74680..74802 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	74381..74588 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN07_RS24895 (69609)	69609..69782	-	174	Protein_83	hypothetical protein	-
LXN07_RS24900 (70084)	70084..70605	+	522	WP_012881138.1	single-stranded DNA-binding protein	-
LXN07_RS24905 (70661)	70661..70894	+	234	WP_000005971.1	DUF905 domain-containing protein	-
LXN07_RS24910 (70958)	70958..72916	+	1959	WP_257120052.1	ParB/RepB/Spo0J family partition protein	-
LXN07_RS24915 (72971)	72971..73405	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
LXN07_RS24920 (73402)	73402..74121	+	720	WP_012881136.1	plasmid SOS inhibition protein A	-
LXN07_RS24925 (74118)	74118..74432	+	315	WP_000872086.1	hypothetical protein	-
- (74381)	74381..74588	+	208	NuclAT_0	-	Antitoxin
- (74381)	74381..74588	+	208	NuclAT_0	-	Antitoxin
- (74381)	74381..74588	+	208	NuclAT_0	-	Antitoxin
- (74381)	74381..74588	+	208	NuclAT_0	-	Antitoxin
LXN07_RS24930 (74622)	74622..74735	+	114	Protein_90	DUF5431 family protein	-
LXN07_RS24935 (74680)	74680..74802	+	123	WP_223412041.1	Hok/Gef family protein	Toxin
LXN07_RS24940 (75103)	75103..75352	-	250	Protein_92	hypothetical protein	-
LXN07_RS24945 (75648)	75648..75971	+	324	WP_000533253.1	hypothetical protein	-
LXN07_RS24950 (76030)	76030..76272	+	243	WP_000540588.1	hypothetical protein	-
LXN07_RS24955 (76472)	76472..76624	-	153	Protein_95	hypothetical protein	-
LXN07_RS24960 (76737)	76737..78404	-	1668	WP_117313998.1	group II intron reverse transcriptase/maturase	-
LXN07_RS24965 (79133)	79133..79306	-	174	Protein_97	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..97598	97598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4560.33 Da
• Isoelectric Point: 8.2774

>T230212 WP_223412041.1 NZ_CP090197:74680-74802 [Escherichia coli]
IVCCTLLIFTHLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T230212 NZ_CP124777:1266441-1266710 [Lachnoanaerobaculum gingivalis]
ATGATTTATGAAATAAGTACAACAGAAAAATTTGATAAAGCATTAAAAAAATTAGATAGGCAAACTCAAAGGATAATCAA
AGCATGGATTGAAAAAAATCTGATAAACTGTGAAAATCCAAGAATACACGGTAAAGCTCTCACCGCAAATAGGAGTGGGC
AGTGGAGGTACCGAGTTGGAGACTATCGTATTTTAGCTGAAATACATGATAATAAACTTGTATTAATTCTCATTGATGTT
GGACATAGAAAAGATATTTATTTGTTTTAA

Antitoxin

Nucleotide

• Length: 208 bp

>AT230212 NZ_CP090197:74381-74588 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin