Detailed information of TA system TA230210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4907364..4907966
• Replicon: chromosome
• Accession: NZ_CP090196
• Organism: Escherichia coli strain YL3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LXN07_RS23505
• Protein ID: WP_000897305.1
• Coordinates: 4907655..4907966 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LXN07_RS23500
• Protein ID: WP_000356395.1
• Coordinates: 4907364..4907654 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN07_RS23470 (4902987)	4902987..4903889	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LXN07_RS23475 (4903886)	4903886..4904521	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LXN07_RS23480 (4904518)	4904518..4905447	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
LXN07_RS23485 (4905629)	4905629..4905871	-	243	WP_021523315.1	hypothetical protein	-
LXN07_RS23490 (4906090)	4906090..4906308	-	219	WP_001295676.1	CopG family transcriptional regulator	-
LXN07_RS23495 (4906727)	4906727..4907005	-	279	WP_001306650.1	hypothetical protein	-
LXN07_RS23500 (4907364)	4907364..4907654	-	291	WP_000356395.1	NadS family protein	Antitoxin
LXN07_RS23505 (4907655)	4907655..4907966	-	312	WP_000897305.1	hypothetical protein	Toxin
LXN07_RS23510 (4908195)	4908195..4909103	+	909	WP_001306651.1	alpha/beta hydrolase	-
LXN07_RS23515 (4909272)	4909272..4910186	-	915	WP_077634137.1	transposase	-
LXN07_RS23520 (4910199)	4910199..4911086	-	888	Protein_4597	hypothetical protein	-
LXN07_RS23525 (4911502)	4911502..4912443	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LXN07_RS23530 (4912488)	4912488..4912925	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T230210 WP_000897305.1 NZ_CP090196:c4907966-4907655 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T230210 NZ_CP124754:4462971-4463261 [Serratia sp. K-E0102]
ATGATTAAATCTTTCCGCGATCGCTACCTGGAGCAGTTCTATCTGGGAGGCAGACGCAGCCGTCTGATCCCCGGTGCGCT
CGAGCGGCAGTTGGCCAGAAAACTCGATATGTTGGCCGCCGCGCAGCAAGAGCGTGATTTGCATCACCCCACGGGTAACT
ATTACAAACGGCTATCCGGGCCATTTCAGGGATGGTCAAGCCTGCGCGTCAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCTGAAAAAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT230210 WP_000356395.1 NZ_CP090196:c4907654-4907364 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT230210 NZ_CP124754:4463276-4463599 [Serratia sp. K-E0102]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGAGAAGTGCTGCAGGAAGAATATTTAACGCCGCTAGGCATCGG
CCAGGAGGAACTGGCAAAGGTCTTGGGCGTCACCCGCGCCACCGTCAATCGCCTGTGCAACGGGCATCGACGCATGAGCG
TTGCCGAGGCCAATCTGTTTGCCGATCTGTTCGAAACGACCGCTGACTTTTGGCTAAACCTGCAGGCGGCACACGATCGC
TGGGAAGCCAGACAGTCCGATGCGCAGCGTGTGCGGCTGCGGCCAATCAAAGAAATACTGGGGCGCAATATCACTCATGC
ATGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7