Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4022212..4022906 Replicon chromosome
Accession NZ_CP090196
Organism Escherichia coli strain YL3

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag LXN07_RS19340 Protein ID WP_001521903.1
Coordinates 4022212..4022610 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LXN07_RS19345 Protein ID WP_000554758.1
Coordinates 4022613..4022906 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (4018042) 4018042..4018122 - 81 NuclAT_11 - -
- (4018042) 4018042..4018122 - 81 NuclAT_11 - -
- (4018042) 4018042..4018122 - 81 NuclAT_11 - -
- (4018042) 4018042..4018122 - 81 NuclAT_11 - -
LXN07_RS19310 (4017382) 4017382..4018626 - 1245 WP_000189541.1 esterase FrsA -
LXN07_RS19315 (4018718) 4018718..4019176 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
LXN07_RS19320 (4019437) 4019437..4020894 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
LXN07_RS19325 (4020951) 4020951..4021303 - 353 Protein_3785 peptide chain release factor H -
LXN07_RS19330 (4021299) 4021299..4021505 - 207 Protein_3786 RtcB family protein -
LXN07_RS19335 (4021750) 4021750..4022202 - 453 WP_023144376.1 GNAT family N-acetyltransferase -
LXN07_RS19340 (4022212) 4022212..4022610 - 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LXN07_RS19345 (4022613) 4022613..4022906 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LXN07_RS19350 (4022958) 4022958..4024013 - 1056 WP_001226177.1 DNA polymerase IV -
LXN07_RS19355 (4024084) 4024084..4024869 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
LXN07_RS19360 (4024841) 4024841..4026553 + 1713 Protein_3792 flagellar biosynthesis protein FlhA -
LXN07_RS19365 (4026632) 4026632..4026985 + 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
LXN07_RS19370 (4027042) 4027042..4027791 - 750 WP_016233951.1 C40 family peptidase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4012149..4022906 10757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T230205 WP_001521903.1 NZ_CP090196:c4022610-4022212 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T230205 NZ_CP124754:c3105219-3104821 [Serratia sp. K-E0102]
ATGTTCAGTCATATGCTGGACACCAACATTGTTATCTATGTCATTAAGCGCCGGCCGCTGGAAGTGCTGGAGGCATTCAA
TCGTTATGCAGGTAAAATGGTCATTTCGTCAATCACCTATGGCGAATTGGTTCACGGGGTGGAGAAAAGCGCTCGCCCAG
CGGTGAACGCCAGAGTGGTTGAGGATTTTGTCTCCCGATTGGACATTCTCGATTACAGCGCCAAAGCGGCATCGCACTAC
GGCAACATCCGGGCTGCGCTGGAACGCCAGGGAACGCCTATTGGCGTAAACGATCTACATATCGCCGGGCATGCGCGCAG
CGAAGGATTGATACTGGTGACCAATAATCGACGCGAATTTGAGCGCGTTGAGGGTTTGCGGCTGGAAAATTGGTTGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT230205 WP_000554758.1 NZ_CP090196:c4022906-4022613 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT230205 NZ_CP124754:c3105452-3105219 [Serratia sp. K-E0102]
ATGACGTTAGGTTCTGTCTTTACCAATAATCGGACACAGGCTGTACGCTTGCCTGCAGAGGTGCGTTTCCCTGATACCGT
TTCTAAAGTGACCGTCCGCGTAGTGGGCAAAGAGCGCATTCTTGCCCCCCTGGAGAATACCTGGGACAGTTTCTTCTGTG
CGGAAAATGAGGTGAGCGATGATTTCCTCAATGAACGTGCCGGGCAGCACCAGCGCGAGCGGGAAGATTTTTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References