Detailed information of TA system TA230188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1225939..1226666
• Replicon: chromosome
• Accession: NZ_CP090196
• Organism: Escherichia coli strain YL3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: LXN07_RS05845
• Protein ID: WP_001521139.1
• Coordinates: 1225939..1226250 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LXN07_RS05850
• Protein ID: WP_000126294.1
• Coordinates: 1226247..1226666 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN07_RS05820 (1221874)	1221874..1223583	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
LXN07_RS05825 (1223593)	1223593..1224135	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
LXN07_RS05830 (1224135)	1224135..1224902	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
LXN07_RS05835 (1224899)	1224899..1225309	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
LXN07_RS05840 (1225302)	1225302..1225772	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
LXN07_RS05845 (1225939)	1225939..1226250	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
LXN07_RS05850 (1226247)	1226247..1226666	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
LXN07_RS05855 (1226745)	1226745..1228169	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
LXN07_RS05860 (1228178)	1228178..1229635	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
LXN07_RS05865 (1229895)	1229895..1230905	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
LXN07_RS05870 (1231054)	1231054..1231581	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T230188 WP_001521139.1 NZ_CP090196:1225939-1226250 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T230188 NZ_CP124750:550896-551246 [Serratia sp. K-M0706]
ATGTGGACGGTAATGACAACGGAAGAGTTTGATCGGTGGCTCTGTGAACAGGATGAATCGACGCAGGAAAAGGTGTTGGC
CGCGCTGGTGATGCTCGAGCGTGCAGGGCCTTCTCTTCGGCGGCCTTTTGTCGACGTGCTGAAAGGCTCGTTGCATTCCA
ACATGAAAGAGCTGCGTATTCAGCACAAAGGGCGGCCGATTCGCGCTTTTTTCGCCTTTGATCCGGCGCGGCAGGCGATT
GTGCTGTGTGCGGGCGACAAAACGGGTAATGAGAAGCGGTTTTATAAAGTGATGCTGTCCATCGCGGATGCGCAGTTCAC
CCAATATTTGATGTGTTATTTCAAGGAGTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT230188 WP_000126294.1 NZ_CP090196:1226247-1226666 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT230188 NZ_CP124750:551250-551549 [Serratia sp. K-M0706]
ATGAGCACCTTAAAGCAACTGCTGGCCTCCCGCACCCCGGAGAGCCGCCGGCGCATCGAGGAAATGGCCGAAGAGATGCT
GTTTGAAGCGCACCTGCACCAGCTGCGGGAAGCGCTGAATATTTCCCAACAGGAACTGGCGAAAACGCTGGGTATTTCCC
AGCCTTCTGTCGCGGCCATTGAAGGGCGCGGCAATGAGCTGAAGATTTCGACCCTGAAACGCTACGTGGAAGCGATGGGC
GGCACGCTGCGCCTGGACGTTGAGCTGCCGGGCGGCAAGCACGTCGGGTTTACGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9