Detailed information of TA system TA230182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 627680..628479
• Replicon: chromosome
• Accession: NZ_CP090196
• Organism: Escherichia coli strain YL3

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: LXN07_RS02990
• Protein ID: WP_000347272.1
• Coordinates: 627680..628144 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: LXN07_RS02995
• Protein ID: WP_001307405.1
• Coordinates: 628144..628479 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN07_RS02960 (622681)	622681..623115	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
LXN07_RS02965 (623133)	623133..624011	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
LXN07_RS02970 (624001)	624001..624780	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
LXN07_RS02975 (624791)	624791..625264	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
LXN07_RS02980 (625287)	625287..626567	-	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
LXN07_RS02985 (626816)	626816..627625	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
LXN07_RS02990 (627680)	627680..628144	-	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
LXN07_RS02995 (628144)	628144..628479	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
LXN07_RS03000 (628628)	628628..630199	-	1572	WP_001273738.1	galactarate dehydratase	-
LXN07_RS03005 (630574)	630574..631908	+	1335	WP_089502604.1	galactarate/glucarate/glycerate transporter GarP	-
LXN07_RS03010 (631924)	631924..632694	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	627680..639131	11451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T230182 WP_000347272.1 NZ_CP090196:c628144-627680 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T230182 NZ_CP124744:2230870-2231055 [Proteus mirabilis]
TTGAAAAGTTCGGAACTGATTAAGTTGTTAGAAAAAAACGGATGGATACTTGATAGAATAAAAGGAAGTCATCATCAATT
TACTCACCCTGATTTTTCTTTTGTTGTAACAGTACCGCATCCAAGAAAAGATTTAAAAAAAGGAACATTAAATCAAATTA
TTAAAAGTGCCAAACTGAAAAATTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT230182 WP_001307405.1 NZ_CP090196:c628479-628144 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT230182 NZ_CP124744:2231102-2231515 [Proteus mirabilis]
ATGTTATATCCAGCCTTTATTGAAATTGATGAAGATGGTAGCGCAAGTGGCTGGTTCCCTGATATTGATGGTTGCATCTT
TGCGGGGAACAGTATTGAAGAAGCTCACGCAGATGCGAGGAGCGCTATTGATGCCCATTTTGAATTGTTAAGCGAGAAGG
GTTTAGATATCCCCTCTCCAAAATCTCAACAAGAGCATTTAATTAATTCTAGTGGTGATTACAATAATGGAATATGGCTT
TTAGTTGATGTTGATATGGATAAATACGACGGACGAGCAGAGCGTATTAATATCACATTGCCACATCGATTATTGAGTCG
CATCGATACTATCGTAAAAACAAATCCTGAATATGGGAGTCGTAGCGGATTTATTGCCACAGCAACCCGTAAAGAACTCC
AAAAGAATATTTAA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7