Detailed information of TA system TA230165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 849..1500
• Replicon: plasmid pAB1343
• Accession: NZ_CP090183
• Organism: Acinetobacter baumannii strain AB1343

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: LXM98_RS18885
• Protein ID: WP_000269904.1
• Coordinates: 1144..1500 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LXM98_RS18880
• Protein ID: WP_001129974.1
• Coordinates: 849..1151 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM98_RS18875 (LXM98_18875)	647..856	-	210	WP_000069474.1	hypothetical protein	-
LXM98_RS18880 (LXM98_18880)	849..1151	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
LXM98_RS18885 (LXM98_18885)	1144..1500	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LXM98_RS18890 (LXM98_18890)	2219..2362	-	144	WP_233908604.1	hypothetical protein	-
LXM98_RS18895 (LXM98_18895)	2519..3088	-	570	WP_233908606.1	plasmid replication DNA-binding protein	-
LXM98_RS18900 (LXM98_18900)	3166..4096	-	931	Protein_6	replication initiation protein RepM	-
LXM98_RS18905 (LXM98_18905)	4693..5862	-	1170	WP_002134206.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11179	11179

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T230165 WP_000269904.1 NZ_CP090183:c1500-1144 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T230165 NZ_CP124740:c2212123-2211950 [Escherichia coli O157:H7 str. EDL933]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT230165 WP_001129974.1 NZ_CP090183:c1151-849 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT230165 NZ_CP124740:c2212447-2212151 [Escherichia coli O157:H7 str. EDL933]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: N8YFZ1