Detailed information of TA system TA229988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 560305..560807
• Replicon: chromosome
• Accession: NZ_CP090065
• Organism: Pectobacterium polonicum strain BY21311

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LW347_RS02575
• Protein ID: WP_258883903.1
• Coordinates: 560305..560559 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A7W2E625
• Locus tag: LW347_RS02580
• Protein ID: WP_039324900.1
• Coordinates: 560556..560807 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LW347_RS02555 (LW347_02555)	555426..556688	-	1263	WP_258883899.1	L-rhamnose isomerase	-
LW347_RS02560 (LW347_02560)	556685..558175	-	1491	WP_258883900.1	rhamnulokinase	-
LW347_RS02565 (LW347_02565)	558544..559383	+	840	WP_258883901.1	HTH-type transcriptional activator RhaS	-
LW347_RS02570 (LW347_02570)	559408..560262	+	855	WP_258883902.1	helix-turn-helix domain-containing protein	-
LW347_RS02575 (LW347_02575)	560305..560559	-	255	WP_258883903.1	Txe/YoeB family addiction module toxin	Toxin
LW347_RS02580 (LW347_02580)	560556..560807	-	252	WP_039324900.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
LW347_RS02585 (LW347_02585)	560883..562580	-	1698	WP_258883904.1	ABC transporter ATP-binding protein	-
LW347_RS02590 (LW347_02590)	562577..563443	-	867	WP_258883905.1	ABC transporter permease	-
LW347_RS02595 (LW347_02595)	563436..564395	-	960	WP_258883906.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10273.85 Da
• Isoelectric Point: 8.0353

>T229988 WP_258883903.1 NZ_CP090065:c560559-560305 [Pectobacterium polonicum]
VKLIWSEEVWEDYLYWQDIDKRMVKKINELIKETRRTPFEGKGKPEPLKHNLAGFWSRRITEEHRLVYAITDDSMMIAAC
RYHY

Nucleotide

• Length: 255 bp

>T229988 NZ_CP124655:c4360023-4359730 [Pseudomonas aeruginosa]
GTGCCACGACTGATTGTCACTGAAGGCGCGGCGAAGGGCTTGGAGCGTTGCCGGCGATTCCTCTCCGATAAAGATCCGCA
AGTCGCGCGCCGCGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGCCCATTTC
CAGATCTGCCTGAGCTGCGTGAGCTCATCATCGAGTTTGGGGACTCAGGTTACGTCGCGCTGTATCGCTATGAGCGCGCA
GATGACACAGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9367.64 Da
• Isoelectric Point: 4.6119

>AT229988 WP_039324900.1 NZ_CP090065:c560807-560556 [Pectobacterium polonicum]
MRTISYSEARQNLSATMMKTVEDRAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANAKRLMNSIESLKAGNGEERD
IIE

Nucleotide

• Length: 252 bp

>AT229988 NZ_CP124655:c4360283-4360011 [Pseudomonas aeruginosa]
ATGGCCACTTCCATCAAAATTGACGACGAGCTGAAAGGTCGCATTCAGCATTTGGCTGGTTTGCGGCAGCGTTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGCGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCGTATCAGGAAACCGGCAGGCATTTGACCGGCCAAGAGGCTCGCACCTGGCTTGGTAGCTGGGGTACAGAT
GCTGAGGCAGAGCTTCCCAAGTGCCACGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7W2E625