Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3687889..3688535 Replicon chromosome
Accession NZ_CP090061
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP-NE368

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag LRM38_RS18240 Protein ID WP_002920560.1
Coordinates 3687889..3688236 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LRM38_RS18245 Protein ID WP_002920557.1
Coordinates 3688236..3688535 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LRM38_RS18230 (LRM38_18215) 3683815..3685248 + 1434 WP_002920564.1 glycogen synthase GlgA -
LRM38_RS18235 (LRM38_18220) 3685266..3687713 + 2448 WP_002920561.1 glycogen phosphorylase -
LRM38_RS18240 (LRM38_18225) 3687889..3688236 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LRM38_RS18245 (LRM38_18230) 3688236..3688535 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LRM38_RS18250 (LRM38_18235) 3688598..3690106 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LRM38_RS18255 (LRM38_18240) 3690311..3690640 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LRM38_RS18260 (LRM38_18245) 3690691..3691521 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
LRM38_RS18265 (LRM38_18250) 3691571..3692329 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T229970 WP_002920560.1 NZ_CP090061:3687889-3688236 [Klebsiella pneumoniae subsp. pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T229970 NZ_CP124652:1401787-1402086 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT229970 WP_002920557.1 NZ_CP090061:3688236-3688535 [Klebsiella pneumoniae subsp. pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT229970 NZ_CP124652:1402083-1402373 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGATAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References