Detailed information of TA system TA229924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 363719..364347
• Replicon: chromosome
• Accession: NZ_CP090028
• Organism: Serratia ureilytica strain W051

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8G2G488
• Locus tag: LSG14_RS01735
• Protein ID: WP_016930174.1
• Coordinates: 364057..364347 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B4GQY7
• Locus tag: LSG14_RS01730
• Protein ID: WP_015379150.1
• Coordinates: 363719..364042 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG14_RS01705	358730..359965	-	1236	WP_028128305.1	serine/threonine transporter SstT	-
LSG14_RS01710	360229..361194	-	966	WP_187048946.1	TerC family protein	-
LSG14_RS01715	361450..362181	-	732	WP_033644531.1	glutamine amidotransferase	-
LSG14_RS01720	362183..363163	-	981	WP_072626939.1	Gfo/Idh/MocA family oxidoreductase	-
LSG14_RS01725	363214..363726	-	513	WP_042785417.1	M48 family metallopeptidase	-
LSG14_RS01730	363719..364042	-	324	WP_015379150.1	HigA family addiction module antitoxin	Antitoxin
LSG14_RS01735	364057..364347	-	291	WP_016930174.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LSG14_RS01740	364578..365717	+	1140	WP_033644533.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
LSG14_RS01745	365912..367933	-	2022	WP_187048412.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-71)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11645.30 Da
• Isoelectric Point: 10.2782

>T229924 WP_016930174.1 NZ_CP090028:c364347-364057 [Serratia ureilytica]
MIKSFRDRYLEQFYLEGRRSRLIPGALERQLARKLDMLAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAEKIYLDPHQDT

Nucleotide

• Length: 291 bp

>T229924 NZ_CP124638:c6091659-6091369 [Pseudomonas aeruginosa]
ATGTACTCCATTATCGAAACCGAGGCGTTCAGCGACTGGCTGAACGGCCTGAAAGATACGGTCACCCGCATGCGCTTGAT
CAAGCGTTTGCAGCGCGCAAGCCTGGGCAACCTGGGCGATGTGAAGCCGGTTGGTGAAGGGGTGTATGAAATGCGCGAGT
TTTTCGGCCCTGGCTGGCGCATGTACTACGTGCAGCAGGGCGATGTGCTGCTGATCATGCTGGGCGGTGGCGACAAATCC
AGCCAGCAGCGTGATATCGAGCGGGCAATTCAGTTGTCCAAGGAGCTCTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12141.78 Da
• Isoelectric Point: 6.2285

>AT229924 WP_015379150.1 NZ_CP090028:c364042-363719 [Serratia ureilytica]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Nucleotide

• Length: 324 bp

>AT229924 NZ_CP124638:c6091368-6091075 [Pseudomonas aeruginosa]
ATGAACAAGACCAAGCTGAACATGCGGCCCTTCGATGCGGCCCACTACTTGAACAATGAGGAAGAGATGGCCGCCTACCT
CGACGTGATTCTGGAAGACAACGACCCGGCGCTGCTGGCCGTTGCTCTGGGCGATATCGCCCGTGCACGCGGCATGACCG
AGCTGGCCCGTAAAACCGGCCTGTCGCGTGAGAGCCTGTACAAGAGCCTGTCCGGCGAGCGCGTGCCGAGCACTGAAACG
CTGCTGAAGGTCATTCATGCCCTGGGCTTCAAGCTGACGGTCTCCGCCGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GQY7