Detailed information of TA system TA229923

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 300362..301092
• Replicon: chromosome
• Accession: NZ_CP090028
• Organism: Serratia ureilytica strain W051

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: LSG14_RS01415
• Protein ID: WP_004937033.1
• Coordinates: 300781..301092 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LSG14_RS01410
• Protein ID: WP_182074436.1
• Coordinates: 300362..300784 (-)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSG14_RS01395	296345..297085	+	741	WP_047573565.1	KDGP aldolase family protein	-
LSG14_RS01400	297173..298387	+	1215	WP_233788328.1	lactonase family protein	-
LSG14_RS01405	298439..300355	+	1917	WP_033644486.1	PRD domain-containing protein	-
LSG14_RS01410	300362..300784	-	423	WP_182074436.1	helix-turn-helix domain-containing protein	Antitoxin
LSG14_RS01415	300781..301092	-	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
LSG14_RS01420	301387..301659	-	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
LSG14_RS01425	301656..302510	-	855	WP_015379177.1	RNase adapter RapZ	-
LSG14_RS01430	302638..303099	-	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
LSG14_RS01435	303255..303542	-	288	WP_004937038.1	ribosome hibernation promoting factor	-
LSG14_RS01440	303566..304999	-	1434	WP_004937039.1	RNA polymerase factor sigma-54	-
LSG14_RS01445	305052..305777	-	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T229923 WP_004937033.1 NZ_CP090028:c301092-300781 [Serratia ureilytica]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T229923 NZ_CP124638:c6091368-6091075 [Pseudomonas aeruginosa]
ATGAACAAGACCAAGCTGAACATGCGGCCCTTCGATGCGGCCCACTACTTGAACAATGAGGAAGAGATGGCCGCCTACCT
CGACGTGATTCTGGAAGACAACGACCCGGCGCTGCTGGCCGTTGCTCTGGGCGATATCGCCCGTGCACGCGGCATGACCG
AGCTGGCCCGTAAAACCGGCCTGTCGCGTGAGAGCCTGTACAAGAGCCTGTCCGGCGAGCGCGTGCCGAGCACTGAAACG
CTGCTGAAGGTCATTCATGCCCTGGGCTTCAAGCTGACGGTCTCCGCCGCTTGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15770.10 Da
• Isoelectric Point: 5.2060

>AT229923 WP_182074436.1 NZ_CP090028:c300784-300362 [Serratia ureilytica]
MITDAIKAADALLRAVPLLQGSHAQQDYRDALELVEYLLENDERHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT229923 NZ_CP124638:c6091659-6091369 [Pseudomonas aeruginosa]
ATGTACTCCATTATCGAAACCGAGGCGTTCAGCGACTGGCTGAACGGCCTGAAAGATACGGTCACCCGCATGCGCTTGAT
CAAGCGTTTGCAGCGCGCAAGCCTGGGCAACCTGGGCGATGTGAAGCCGGTTGGTGAAGGGGTGTATGAAATGCGCGAGT
TTTTCGGCCCTGGCTGGCGCATGTACTACGTGCAGCAGGGCGATGTGCTGCTGATCATGCTGGGCGGTGGCGACAAATCC
AGCCAGCAGCGTGATATCGAGCGGGCAATTCAGTTGTCCAAGGAGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63