Detailed information of TA system TA229916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 742834..743346
• Replicon: chromosome
• Accession: NZ_CP090007
• Organism: Streptococcus salivarius strain SALI-10

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: W3XTH0
• Locus tag: LV497_RS03015
• Protein ID: WP_014634942.1
• Coordinates: 742834..743088 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: LV497_RS03020
• Protein ID: WP_013991075.1
• Coordinates: 743092..743346 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LV497_RS02990 (LV497_02990)	738055..738354	+	300	WP_022496188.1	hypothetical protein	-
LV497_RS02995 (LV497_02995)	738371..738721	-	351	WP_183139907.1	ribonuclease	-
LV497_RS03000 (LV497_03000)	738759..739103	-	345	WP_155123768.1	hypothetical protein	-
LV497_RS03005 (LV497_03005)	739122..739619	-	498	WP_245085241.1	hypothetical protein	-
LV497_RS03010 (LV497_03010)	740013..742118	-	2106	WP_245085244.1	ATP-dependent Clp protease ATP-binding subunit	-
LV497_RS03015 (LV497_03015)	742834..743088	-	255	WP_014634942.1	Txe/YoeB family addiction module toxin	Toxin
LV497_RS03020 (LV497_03020)	743092..743346	-	255	WP_013991075.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LV497_RS03025 (LV497_03025)	743488..744579	-	1092	WP_245085247.1	nucleotidyltransferase	-
LV497_RS03030 (LV497_03030)	744586..745326	-	741	WP_179969005.1	class I SAM-dependent methyltransferase	-
LV497_RS03035 (LV497_03035)	745531..745884	-	354	WP_084869239.1	ribosome silencing factor	-
LV497_RS03040 (LV497_03040)	745907..746497	-	591	WP_084869237.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LV497_RS03045 (LV497_03045)	746494..747126	-	633	WP_002884372.1	nicotinate-nucleotide adenylyltransferase	-
LV497_RS03050 (LV497_03050)	747234..747551	-	318	WP_002884193.1	ribosome assembly RNA-binding protein YhbY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-75)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10111.55 Da
• Isoelectric Point: 8.8499

>T229916 WP_014634942.1 NZ_CP090007:c743088-742834 [Streptococcus salivarius]
MLLTFTEDAWTDYCYWQNQDRKTLKRINKLIKDIQRDPFSGIGKPEPLKYDFQGAWSRRIDAENRLIYMIDGKSVAFLSF
KDHY

Nucleotide

• Length: 255 bp

>T229916 NZ_CP124633:49883-50173 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9719.00 Da
• Isoelectric Point: 4.8782

>AT229916 WP_013991075.1 NZ_CP090007:c743346-743092 [Streptococcus salivarius]
MEAVVYSTFRNHLKDYMKKVNDEFEPLAVVNKNPDEDIVVVSKSEWDSIQETLRIVQNKELSDKVLRGMAQVRAGETQEH
AIKE

Nucleotide

• Length: 255 bp

>AT229916 NZ_CP124633:49587-49886 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCTAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATTCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Toxin

• AlphaFold DB: W3XTH0