Detailed information of TA system TA229881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 69935..70662
• Replicon: plasmid p3IncF
• Accession: NZ_CP089990
• Organism: Klebsiella pneumoniae strain LZKP00003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LUR00_RS26105
• Protein ID: WP_011251285.1
• Coordinates: 69935..70246 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LUR00_RS26110
• Protein ID: WP_011251286.1
• Coordinates: 70243..70662 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUR00_RS26075 (LUR00_26075)	65126..66106	+	981	WP_000019441.1	IS5-like element ISKpn26 family transposase	-
LUR00_RS26080 (LUR00_26080)	66652..66831	-	180	Protein_71	IS5/IS1182 family transposase	-
LUR00_RS26085 (LUR00_26085)	66871..68409	-	1539	WP_017901237.1	IS66 family transposase	-
LUR00_RS26090 (LUR00_26090)	68458..68805	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
LUR00_RS26095 (LUR00_26095)	68802..69179	-	378	WP_004118218.1	transposase	-
LUR00_RS26100 (LUR00_26100)	69293..69730	+	438	Protein_75	DDE-type integrase/transposase/recombinase	-
LUR00_RS26105 (LUR00_26105)	69935..70246	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LUR00_RS26110 (LUR00_26110)	70243..70662	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LUR00_RS26115 (LUR00_26115)	70732..71145	+	414	WP_017901337.1	hypothetical protein	-
LUR00_RS26120 (LUR00_26120)	71191..72158	-	968	Protein_79	IS5-like element IS903B family transposase	-
LUR00_RS26125 (LUR00_26125)	72262..72528	+	267	WP_017900868.1	hypothetical protein	-
LUR00_RS26130 (LUR00_26130)	72642..73177	-	536	Protein_81	transposase	-
LUR00_RS26135 (LUR00_26135)	73702..75069	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-14 / sul1 / blaDHA-1 / qnrB4	-	1..190701	190701
	-	inside	IScluster/Tn	-	-	65126..72158	7032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T229881 WP_011251285.1 NZ_CP089990:69935-70246 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T229881 NZ_CP124622:c2904017-2903439 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCTGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGTTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGTTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT229881 WP_011251286.1 NZ_CP089990:70243-70662 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT229881 NZ_CP124622:c2904660-2904106 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1