Detailed information of TA system TA022988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 2329312..2329970
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B4SDR9
• Locus tag: PPHA_RS11085
• Protein ID: WP_012508913.1
• Coordinates: 2329312..2329635 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PPHA_RS11090
• Protein ID: WP_012508914.1
• Coordinates: 2329632..2329970 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS11075	2325694..2327409	-	1716	WP_012508911.1	site-specific DNA-methyltransferase	-
PPHA_RS11080	2327643..2328968	-	1326	WP_012508912.1	transcriptional regulator	-
PPHA_RS11085	2329312..2329635	+	324	WP_012508913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPHA_RS11090	2329632..2329970	+	339	WP_012508914.1	helix-turn-helix transcriptional regulator	Antitoxin
PPHA_RS15690	2330032..2330280	-	249	WP_012508915.1	hypothetical protein	-
PPHA_RS16015	2330315..2330602	+	288	WP_190273992.1	hypothetical protein	-
PPHA_RS11100	2330702..2331082	+	381	WP_012508917.1	MbcA/ParS/Xre antitoxin family protein	-
PPHA_RS11105	2331042..2331776	+	735	WP_012508918.1	RES family NAD+ phosphorylase	-
PPHA_RS11110	2331923..2332633	+	711	WP_012508919.1	pyridoxine 5'-phosphate synthase	-
PPHA_RS11115	2332642..2333346	+	705	WP_012508920.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
PPHA_RS11120	2333343..2334191	+	849	WP_012508921.1	undecaprenyl-diphosphatase UppP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-100)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12161.43 Da
• Isoelectric Point: 10.7466

>T22988 WP_012508913.1 NC_011060:2329312-2329635 [Pelodictyon phaeoclathratiforme BU-1]
MSYQILFFNKSVQKEIEAWPIGINASFVRITEQIVISGPNLGLPYTRPFGDGLFEIRARGAEGIGRAFFCCLVDRKVVIL
HGFIKKRQATPIKELKLARKRLKEITP

Nucleotide

• Length: 324 bp

>T22988 NC_011060:2329312-2329635 [Pelodictyon phaeoclathratiforme BU-1]
ATGAGCTATCAAATTCTGTTTTTCAATAAATCCGTCCAGAAAGAGATTGAAGCCTGGCCAATCGGCATCAATGCGAGCTT
TGTCCGGATTACAGAGCAAATCGTGATCTCTGGGCCAAATCTTGGGTTACCCTACACCCGACCATTTGGTGATGGATTGT
TCGAAATTCGTGCAAGAGGTGCTGAGGGAATTGGACGAGCTTTTTTCTGTTGTCTGGTTGACCGGAAGGTTGTCATTTTG
CATGGATTCATAAAAAAGAGACAGGCGACACCAATCAAAGAGTTGAAACTTGCCAGAAAACGGCTGAAGGAGATTACACC
ATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12129.80 Da
• Isoelectric Point: 8.0470

>AT22988 WP_012508914.1 NC_011060:2329632-2329970 [Pelodictyon phaeoclathratiforme BU-1]
MSDSNTYNPVAFDPKAYAAHNSSSDPKFKIAYDALEDEFAALRALLHARTQAGLTQAEVASRMGVSQPVLARIEASLGKQ
DHSPSLNTLRRYANACGMKLVIQMVLPNTPVP

Nucleotide

• Length: 339 bp

>AT22988 NC_011060:2329632-2329970 [Pelodictyon phaeoclathratiforme BU-1]
ATGAGTGACAGCAACACCTATAATCCTGTAGCCTTTGACCCAAAAGCTTATGCGGCTCATAACAGCTCAAGCGACCCAAA
GTTCAAAATAGCCTACGATGCACTTGAAGATGAGTTTGCGGCTCTCAGGGCACTTTTGCATGCCCGTACGCAGGCGGGCC
TCACCCAGGCTGAAGTGGCTTCACGTATGGGAGTTTCGCAGCCTGTTCTGGCACGCATTGAGGCAAGCCTGGGCAAGCAG
GATCACTCTCCATCACTCAATACCTTGCGCCGATATGCAAACGCATGCGGCATGAAACTGGTCATCCAGATGGTTTTGCC
CAATACACCCGTTCCATAG

Structures

Toxin

• AlphaFold DB: B4SDR9