Detailed information of TA system TA229869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2538926..2539572
• Replicon: chromosome
• Accession: NZ_CP089989
• Organism: Klebsiella pneumoniae strain LZKP00003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LUR00_RS12030
• Protein ID: WP_102011763.1
• Coordinates: 2538926..2539273 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8UB68
• Locus tag: LUR00_RS12035
• Protein ID: WP_002920557.1
• Coordinates: 2539273..2539572 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUR00_RS12020 (LUR00_12020)	2534852..2536285	+	1434	WP_102011764.1	glycogen synthase GlgA	-
LUR00_RS12025 (LUR00_12025)	2536303..2538750	+	2448	WP_002920561.1	glycogen phosphorylase	-
LUR00_RS12030 (LUR00_12030)	2538926..2539273	+	348	WP_102011763.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LUR00_RS12035 (LUR00_12035)	2539273..2539572	+	300	WP_002920557.1	helix-turn-helix transcriptional regulator	Antitoxin
LUR00_RS12040 (LUR00_12040)	2539635..2541143	-	1509	WP_002920554.1	glycerol-3-phosphate dehydrogenase	-
LUR00_RS12045 (LUR00_12045)	2541348..2541677	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
LUR00_RS12050 (LUR00_12050)	2541728..2542558	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
LUR00_RS12055 (LUR00_12055)	2542608..2543366	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13535.49 Da
• Isoelectric Point: 5.2054

>T229869 WP_102011763.1 NZ_CP089989:2538926-2539273 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDNDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T229869 NZ_CP124601:c1357895-1357149 [Bacillus stercoris]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATTGTGGCTGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACATGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGATCGGTATCTCATTGTCGCAGTCAGCTTTGCTTTGATTGATGCTTTC
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCTGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGCATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCAATATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAATGGTTCTCATCCCTTTTACCATCGAGACAAAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTACTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10800.58 Da
• Isoelectric Point: 10.1464

>AT229869 WP_002920557.1 NZ_CP089989:2539273-2539572 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT229869 NZ_CP124601:c1357149-1356979 [Bacillus stercoris]
ATGGAACGTGCGTTTCAAAACGGATGCGAGCCCAGAGCGGCCAAACCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCACACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1CBF8