Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 778324..778921 Replicon chromosome
Accession NZ_CP089989
Organism Klebsiella pneumoniae strain LZKP00003

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LUR00_RS03740 Protein ID WP_004142563.1
Coordinates 778604..778921 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LUR00_RS03735 Protein ID WP_004142561.1
Coordinates 778324..778611 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUR00_RS03705 (LUR00_03705) 774404..774652 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LUR00_RS03710 (LUR00_03710) 774670..775011 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LUR00_RS03715 (LUR00_03715) 775042..776157 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
LUR00_RS03720 (LUR00_03720) 776337..776918 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
LUR00_RS03725 (LUR00_03725) 776918..777286 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LUR00_RS03730 (LUR00_03730) 777406..778059 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
LUR00_RS03735 (LUR00_03735) 778324..778611 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LUR00_RS03740 (LUR00_03740) 778604..778921 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LUR00_RS03745 (LUR00_03745) 779106..780149 - 1044 WP_023284821.1 DUF2157 domain-containing protein -
LUR00_RS03750 (LUR00_03750) 780819..781685 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
LUR00_RS03755 (LUR00_03755) 781794..783221 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T229862 WP_004142563.1 NZ_CP089989:c778921-778604 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T229862 NZ_CP124600:c2705669-2705091 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
TCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGTGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT229862 WP_004142561.1 NZ_CP089989:c778611-778324 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT229862 NZ_CP124600:c2706312-2705758 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References