Detailed information of TA system TA229857

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3894191..3894788
• Replicon: chromosome
• Accession: NZ_CP089986
• Organism: Klebsiella pneumoniae strain LZKP00001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: LUA09_RS18880
• Protein ID: WP_004893639.1
• Coordinates: 3894471..3894788 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: LUA09_RS18875
• Protein ID: WP_004142561.1
• Coordinates: 3894191..3894478 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LUA09_RS18845 (LUA09_18845)	3890271..3890519	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
LUA09_RS18850 (LUA09_18850)	3890537..3890878	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
LUA09_RS18855 (LUA09_18855)	3890909..3892024	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
LUA09_RS18860 (LUA09_18860)	3892204..3892785	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
LUA09_RS18865 (LUA09_18865)	3892785..3893153	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
LUA09_RS18870 (LUA09_18870)	3893273..3893926	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
LUA09_RS18875 (LUA09_18875)	3894191..3894478	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
LUA09_RS18880 (LUA09_18880)	3894471..3894788	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LUA09_RS18885 (LUA09_18885)	3894973..3896016	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
LUA09_RS18890 (LUA09_18890)	3896682..3897548	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
LUA09_RS18895 (LUA09_18895)	3897657..3899084	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T229857 WP_004893639.1 NZ_CP089986:c3894788-3894471 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T229857 NZ_CP124576:3073166-3074080 [Sphingobium sp. AP49]
ATGAGCCGGCCGCCGAAGAACATGGCGATCTCGGTGCGCGACCGGCTGACGGCGCGTGCCCGCGAGCGTCGGGAAAACGC
ACAGCTGCTGATGACCCGCTACGTCATCGAACGTCTGCTCTTTCGCCTGAGCCTGTCGCCACATCGCGAGCGGTTCGTGC
TCAAGGGCGCGATGCTGTTCAGCCTGTGGGCGGCAGCGCCTTATCGCGCCACCGGCGACCTCGATCTGCTGGGCATAGGC
GAAAACGCGCCCGAGCAGCTCGCGGCGGTTTTTCAGGAAATCCTCGCGGTCGCGGTCGATGACGACGGCATCGTGTTCCG
GCCCGAAACCCTGCGCGCGGCCGCGGCCCGTGCCGAGGATGAATATGCCGGCGTCCGCATCGACTTCGTCGCCGAACTCG
CGGGCGCGCGCCTGCCGATGCACGTCGATATCGGCTATGGCGATGCGATCACGCCGGGCGCGATCGAGATCGAGTATCCG
TCGCTGCTCGGCCAGCCCACCGCACATCTTCGGGCCTATCCGCCCGAGACGGTCGTGGCCGAGAAATTCCAGGCCATGGT
CGCGCTCGGTATGCTGAACACGCGCCTCAAGGATTTCTACGACATCTGGGCGATCGCGGGATCGTTCGCGTTCGATGGCG
CCGTGCTGTCGCGCGCCATCCAGGCCACCTTCGATCGCCGCCAAACCCCTTTGCCTGGCGAGACGCCCCCGGCGCTCACA
CCCGCCTTCGCTGACGAGAAACAGGCGGAATGGACAGCGTTCCTGCGGCGGACCGAAATCGCGCTGGCGCCAGAACCGTT
CGCGGCGATCCAGGCTCAAGTCGCGACGCTGGTGATGCCGCCGGTACTGGCGCTCGGCCAAGGCGCCGCCTTTGCCGGTG
ACTGGCCCAGCGGTGGTCCTTGGAAGGAGAGGTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT229857 WP_004142561.1 NZ_CP089986:c3894478-3894191 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT229857 NZ_CP124576:3072561-3073169 [Sphingobium sp. AP49]
ATGGAACCGACGACTCATCGAGAACGGGCCCTGGAGGTCGTCCGGCACCGCGGCATTGCGCGCGGACGCGACTTCGACGC
GGCCGGCGTGACGCGGGCGACCCTGCAAAGGCTGCGTGACGAAGGCGTCCTCCAGCAGGTCGGCCGCGGGCTCTACAAAC
TGGCCGACGCGAACGTCGACAGTTCAACGAGCCTGGCCGAGGCCGTCCGCATCCAGCCGCGCGGCGTCATCTGCCTGCTG
TCGGCGCTGCAGTTCCATGAGCTGACCACGCAGACCCCCCATGCGGTGTGGATGATGCTCGGCCAAAAGGATTGGGCGCC
GGTCAATCCGTCCGTGGCGCTGAAGATCGTTCGCGCGAGCGGCGAAGCGCTGACAGCGGGCGTCGAGACACACGTCATCG
ACGGCGTTCCGGTGCCCATCACCGTACCGGCCAAGACCGTCGCCGACTGCTTCAAGCACCGCAACAAGATCGGTATCGAC
GTGGCGGTCGAGGCCCTGCGCGATTTCATGAAGTCCCGGCCCCGGCCGGACCTCAATGCGATCTACCGTTATGCTGAGCT
CGATCGGGTCCAATCGGTGATCCGGCCCTATCTCGAAGCCATGACATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1