Detailed information of TA system TA022985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1832032..1832677
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B4SB12
• Locus tag: PPHA_RS08535
• Protein ID: WP_012508445.1
• Coordinates: 1832032..1832358 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PPHA_RS08540
• Protein ID: WP_012508446.1
• Coordinates: 1832351..1832677 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS08485	1827057..1827290	+	234	WP_041526794.1	hypothetical protein	-
PPHA_RS08490	1827457..1827669	-	213	WP_041526492.1	hypothetical protein	-
PPHA_RS08495	1827650..1827931	-	282	WP_012508438.1	BrnT family toxin	-
PPHA_RS08500	1828073..1828300	-	228	WP_012508439.1	CopG family transcriptional regulator	-
PPHA_RS08505	1828350..1828853	-	504	WP_012508440.1	GNAT family N-acetyltransferase	-
PPHA_RS08510	1828860..1829138	-	279	WP_012508441.1	DUF1778 domain-containing protein	-
PPHA_RS15115	1829228..1830236	-	1009	Protein_1668	ABC transporter permease	-
PPHA_RS08520	1830494..1830883	-	390	WP_012508442.1	type II toxin-antitoxin system VapC family toxin	-
PPHA_RS08525	1830880..1831110	-	231	WP_012508443.1	hypothetical protein	-
PPHA_RS08530	1831362..1831613	-	252	WP_012508444.1	UPF0175 family protein	-
PPHA_RS08535	1832032..1832358	+	327	WP_012508445.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPHA_RS08540	1832351..1832677	+	327	WP_012508446.1	helix-turn-helix domain-containing protein	Antitoxin
PPHA_RS08545	1832737..1832918	-	182	Protein_1674	ABC transporter ATP-binding protein	-
PPHA_RS08550	1833005..1834078	-	1074	WP_012508448.1	metallophosphoesterase family protein	-
PPHA_RS08555	1834336..1835010	+	675	WP_041526494.1	tRNA (guanosine(18)-2'-O)-methyltransferase TrmH	-
PPHA_RS08560	1835039..1836088	-	1050	WP_012508450.1	DUF4332 domain-containing protein	-
PPHA_RS08565	1836202..1836744	-	543	WP_012508451.1	hypothetical protein	-
PPHA_RS08570	1836756..1837178	-	423	WP_041526495.1	rRNA maturation RNase YbeY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1829670..1846281	16611

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-96)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12497.78 Da
• Isoelectric Point: 9.7065

>T22985 WP_012508445.1 NC_011060:1832032-1832358 [Pelodictyon phaeoclathratiforme BU-1]
MAYRIDYFHPRILKEIEGWPESIKTDYARIVELLMDYGHDLRMPCSRAMGNGLFEIRPKGKDGIGRALYCFQTGELIIIP
HAFIKKSQATPRKEMVLALKRMKEVING

Nucleotide

• Length: 327 bp

>T22985 NC_011060:1832032-1832358 [Pelodictyon phaeoclathratiforme BU-1]
ATGGCTTATCGAATTGACTATTTTCACCCTCGGATACTGAAAGAGATTGAGGGTTGGCCGGAGTCGATCAAGACTGATTA
CGCACGCATTGTTGAATTGCTTATGGATTACGGCCATGATTTGCGTATGCCCTGTTCACGGGCAATGGGAAATGGCTTGT
TTGAGATTCGGCCCAAGGGAAAGGATGGCATTGGAAGAGCACTCTACTGTTTTCAGACAGGAGAGCTAATCATTATCCCG
CACGCATTTATCAAGAAATCGCAAGCGACGCCACGAAAAGAGATGGTGCTCGCGCTTAAAAGAATGAAGGAGGTAATCAA
TGGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12128.11 Da
• Isoelectric Point: 10.0517

>AT22985 WP_012508446.1 NC_011060:1832351-1832677 [Pelodictyon phaeoclathratiforme BU-1]
MDDLKYQPVEHNHEEFLRKAMVNKEFKAGYEALGPKYALIRELLAARQRSGMTQEAVALKIGTSKSAISRLESGSKHTPS
VATLRKYADAVGCELEIKLIKKKEPQKI

Nucleotide

• Length: 327 bp

>AT22985 NC_011060:1832351-1832677 [Pelodictyon phaeoclathratiforme BU-1]
ATGGATGATCTCAAATACCAGCCTGTGGAGCACAATCACGAAGAATTTCTCCGCAAGGCAATGGTAAATAAAGAATTTAA
AGCGGGGTATGAGGCTCTTGGACCGAAGTATGCCCTGATACGGGAACTCCTTGCCGCCCGCCAGCGCTCAGGCATGACGC
AAGAAGCTGTTGCTCTGAAAATAGGCACGTCAAAAAGTGCCATCTCCAGACTGGAATCCGGCAGCAAGCATACCCCCTCT
GTTGCCACGCTGAGGAAATACGCCGATGCAGTTGGCTGCGAACTGGAAATCAAACTGATCAAAAAAAAGGAACCGCAAAA
GATCTGA

Structures

Toxin

• AlphaFold DB: B4SB12