Detailed information of TA system TA229823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1186508..1187163
• Replicon: chromosome
• Accession: NZ_CP089973
• Organism: Pseudomonas brassicacearum strain WCS365

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: LRP86_RS05485
• Protein ID: WP_013694185.1
• Coordinates: 1186819..1187163 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRP86_RS05480
• Protein ID: WP_003205391.1
• Coordinates: 1186508..1186822 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRP86_RS05460 (LRP86_01091)	1182554..1183471	+	918	WP_100264014.1	LysR family transcriptional regulator	-
LRP86_RS05465 (LRP86_01092)	1183621..1184634	+	1014	WP_003205388.1	sensor domain-containing diguanylate cyclase	-
LRP86_RS05470 (LRP86_01093)	1184726..1185469	+	744	WP_003205389.1	DUF72 domain-containing protein	-
LRP86_RS05475 (LRP86_01094)	1185591..1186295	+	705	WP_003205390.1	hypothetical protein	-
LRP86_RS05480 (LRP86_01095)	1186508..1186822	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
LRP86_RS05485 (LRP86_01096)	1186819..1187163	-	345	WP_013694185.1	hypothetical protein	Toxin
LRP86_RS05490 (LRP86_01097)	1187303..1188190	-	888	WP_003205393.1	heme o synthase	-
LRP86_RS05495 (LRP86_01098)	1188202..1188537	-	336	WP_003205394.1	cytochrome o ubiquinol oxidase subunit IV	-
LRP86_RS05500 (LRP86_01099)	1188538..1189164	-	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
LRP86_RS05505 (LRP86_01100)	1189168..1191198	-	2031	WP_003205396.1	cytochrome o ubiquinol oxidase subunit I	-
LRP86_RS05510 (LRP86_01101)	1191202..1192149	-	948	WP_003205397.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T229823 WP_013694185.1 NZ_CP089973:c1187163-1186819 [Pseudomonas brassicacearum]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T229823 NZ_CP124528:c7108-6842 [Psychrobacter sp. WB2]
ATGAGATCCATTGAAGTATCCAGTCAGTTTAAGAGAGATGCCAAAAAGAATTATCTAAGCTTGCTCACTCCTGCATGGGG
CGAGGTGCTTAATTGTTTGACGAACGATATACCGCTACCGGCTAAATACAAAGACCATGCGTTGACCGGCAATCATAAAG
GGTTTAGGGATTGCCATATTAAGCCTGATTTGGTGCTTATCTACCGTGTTCAGAGCGATACGGTAGATTTTGTTAGGCTG
GGTAGCCATTCAGAGGTGTTTGATTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT229823 WP_003205391.1 NZ_CP089973:c1186822-1186508 [Pseudomonas brassicacearum]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT229823 NZ_CP124528:c7379-7095 [Psychrobacter sp. WB2]
ATGACTACAACTAATTATAATATACGGCTTGACCAGGATCTGAAAGATAGGTCTTTCTCTGTTCTTGAAAGCTATGGTCT
AACGCCTTCACAAGCCATAAGACTGTTCTTGAACCAAGTAGCCGATACTAATGCGATTCCTCTGTCTTTTGACTATCAAC
AACCTAAGCTCAACGCGCAAACACTTGCTGCTATTGCAGAGGTCGCGAGCGGTAACGGCACTAAGTACGATAGCTTTGGT
GATTTGATGGATGAGCTAGAAGGCGAGGCTGATGAGATCCATTGA

Structures

Toxin

• AlphaFold DB: G8QAX9