Detailed information of TA system TA022979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 582398..582815
• Replicon: chromosome
• Accession: NC_011060
• Organism: Pelodictyon phaeoclathratiforme BU-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PPHA_RS14900
• Protein ID: WP_083765344.1
• Coordinates: 582717..582815 (-)
• Length: 33 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PPHA_RS02980
• Protein ID: WP_012507402.1
• Coordinates: 582398..582724 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PPHA_RS02960	578711..579703	+	993	WP_012507399.1	Rpn family recombination-promoting nuclease/putative transposase	-
PPHA_RS02965	579782..580672	+	891	WP_012507400.1	Rpn family recombination-promoting nuclease/putative transposase	-
PPHA_RS02970	580881..581840	+	960	WP_012507401.1	Rpn family recombination-promoting nuclease/putative transposase	-
PPHA_RS02980	582398..582724	-	327	WP_012507402.1	helix-turn-helix domain-containing protein	Antitoxin
PPHA_RS14900	582717..582815	-	99	WP_083765344.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PPHA_RS02985	583088..584389	-	1302	WP_150085573.1	IS1380 family transposase	-
PPHA_RS14460	584589..584810	-	222	WP_083765345.1	type II toxin-antitoxin system RelE/ParE family toxin	-
PPHA_RS15850	585023..585196	+	174	WP_190274019.1	hypothetical protein	-
PPHA_RS02995	585353..585643	+	291	WP_012507404.1	helix-turn-helix domain-containing protein	-
PPHA_RS03000	585678..586085	+	408	Protein_580	GDP-mannose 4,6-dehydratase	-
PPHA_RS03005	586419..586634	+	216	WP_012507405.1	hypothetical protein	-
PPHA_RS03010	586640..587044	+	405	WP_041526652.1	type II toxin-antitoxin system VapC family toxin	-
PPHA_RS03015	587047..587250	+	204	Protein_583	GDP-mannose 4,6-dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	575422..591166	15744
	-	flank	IS/Tn	-	-	583088..584305	1217

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (1-27)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-96)

Sequences

Toxin

Protein

• Length: 33 a.a.
• Molecular weight: 3684.35 Da
• Isoelectric Point: 11.2211

>T22979 WP_083765344.1 NC_011060:c582815-582717 [Pelodictyon phaeoclathratiforme BU-1]
IIILHTFIKKTQSTPHKELQVARKRLEAVNNG

Nucleotide

• Length: 99 bp

>T22979 NC_011060:c582815-582717 [Pelodictyon phaeoclathratiforme BU-1]
ATTATAATCCTGCATACTTTTATTAAAAAAACTCAGTCAACTCCGCATAAAGAGCTTCAAGTTGCCAGAAAAAGATTAGA
GGCGGTTAACAATGGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12355.18 Da
• Isoelectric Point: 7.8117

>AT22979 WP_012507402.1 NC_011060:c582724-582398 [Pelodictyon phaeoclathratiforme BU-1]
MDDLKYQPVPYNVEEEICKNLQDENFRREYEALEPKYALIRELLSARQLSGMTQEAVALKIGTTKSAISRLESGSKHSPS
LTTLRKYAEAVNCELEIKLKPKQQPKTI

Nucleotide

• Length: 327 bp

>AT22979 NC_011060:c582724-582398 [Pelodictyon phaeoclathratiforme BU-1]
ATGGATGATCTGAAATACCAGCCAGTACCATACAATGTTGAGGAGGAGATATGCAAAAACCTTCAAGACGAGAATTTTCG
CAGAGAATATGAAGCACTGGAACCAAAATACGCATTGATCAGGGAGCTGTTATCTGCACGACAGCTCTCAGGCATGACGC
AAGAAGCTGTGGCTCTGAAAATCGGGACGACCAAAAGTGCCATCTCCAGACTGGAATCCGGCAGCAAGCATTCTCCCTCT
CTTACCACACTGAGAAAATACGCTGAAGCAGTTAATTGCGAACTGGAAATCAAACTGAAACCAAAACAGCAACCGAAAAC
GATCTGA