Detailed information of TA system TA229785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 31941..32592
• Replicon: plasmid pETR001-a
• Accession: NZ_CP089943
• Organism: Erwinia tracheiphila strain MDCuke

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LU604_RS26020
• Protein ID: WP_233480965.1
• Coordinates: 32248..32592 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LU604_RS26015
• Protein ID: WP_233480964.1
• Coordinates: 31941..32255 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LU604_RS25980 (LU604_25980)	27260..28093	-	834	WP_233480958.1	DUF932 domain-containing protein	-
LU604_RS25985 (LU604_25985)	28512..28778	-	267	WP_233480959.1	hypothetical protein	-
LU604_RS25990 (LU604_25990)	28871..29311	-	441	WP_233480960.1	DUF1419 domain-containing protein	-
LU604_RS25995 (LU604_25995)	29277..29435	-	159	WP_233480961.1	hypothetical protein	-
LU604_RS26000 (LU604_26000)	29941..30180	+	240	WP_233480962.1	helix-turn-helix transcriptional regulator	-
LU604_RS26005 (LU604_26005)	30742..30888	-	147	Protein_30	hypothetical protein	-
LU604_RS26010 (LU604_26010)	30945..31523	-	579	WP_233480963.1	antirestriction protein	-
LU604_RS26015 (LU604_26015)	31941..32255	-	315	WP_233480964.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
LU604_RS26020 (LU604_26020)	32248..32592	-	345	WP_233480965.1	toxin	Toxin
LU604_RS26025 (LU604_26025)	32845..33147	-	303	WP_233480966.1	methyltransferase	-
LU604_RS26030 (LU604_26030)	33296..33559	-	264	Protein_35	transposase	-
LU604_RS26035 (LU604_26035)	33648..34580	+	933	WP_233481439.1	IS91 family transposase	-
LU604_RS27155	34537..34872	+	336	WP_267938798.1	hypothetical protein	-
LU604_RS26040 (LU604_26040)	35102..35980	+	879	WP_267938747.1	IS91 family transposase	-
LU604_RS27160	35992..36327	+	336	WP_267938798.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71258	71258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-88)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13723.68 Da
• Isoelectric Point: 9.8634

>T229785 WP_233480965.1 NZ_CP089943:c32592-32248 [Erwinia tracheiphila]
MYALFIELSSFEKYRANYLNDDQFRLFQRMLLADPEKGDLIPDTGGLRKVRYRDERRNKGTRGGIRVIYYWTNEKGQFIL
FTIYDKEQRDDLTKQQRDALAEALSVIKKRLKYE

Nucleotide

• Length: 345 bp

>T229785 NZ_CP124517:2375426-2375803 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12095.76 Da
• Isoelectric Point: 7.3163

>AT229785 WP_233480964.1 NZ_CP089943:c32255-31941 [Erwinia tracheiphila]
MNERDIYSELMTGMQELKDHQDGKITLKTYKVSKREPVTIAPQELRDVREKLNLSQAVFAHYLHTGETTYQNWEQGRARP
NAQAVLLIRMVQQNPETLQTLAQL

Nucleotide

• Length: 315 bp

>AT229785 NZ_CP124517:2374969-2375337 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA